Unraveling the interaction between a glycolytic regulator protein EhPpdk and an anaphase promoting complex protein EhApc10: yeast two hybrid screening, in vitro binding assays and molecular simulation study.

Autor: Pal S; Department of Microbiology, The University of Burdwan, Burdwan, West Bengal, 713104, India., Biswas P; Department of Microbiology, The University of Burdwan, Burdwan, West Bengal, 713104, India., Ghosh R; Department of Microbiology, The University of Burdwan, Burdwan, West Bengal, 713104, India., Dam S; Department of Microbiology, The University of Burdwan, Burdwan, West Bengal, 713104, India. sdam@microbio.buruniv.ac.in.
Jazyk: angličtina
Zdroj: The protein journal [Protein J] 2024 Dec; Vol. 43 (6), pp. 1104-1119. Date of Electronic Publication: 2024 Nov 01.
DOI: 10.1007/s10930-024-10238-5
Abstrakt: The anaphase promoting complex (APC or cyclosome) is a major ubiquitin ligase that coordinates mitotic and G1 progression, acting as a major regulator of chromosome segregation. While the human APC contains fourteen subunits, it is yet to be explored in the pathogen Entamoeba histolytica. Our study reveals the existence of a single functional Apc10 homolog in E. histolytica, which acts as a processivity factor of ubiquitin ligase activity in human. A cDNA library generated from HM1:IMSS strain of E. histolytica was screened for interaction partners of EhApc10 in yeast two hybrid study. The novel interactor, a glycolytic enzyme, pyruvate phosphate dikinase (Ppdk) was found to interact with EhApc10 and further validated by in vitro assay. A comprehensive in silico study has emphasized the structural and functional aspects, encompassing physicochemical traits, predictive 3D structure modelling, validation of EhApc10-EhPpdk interaction through molecular docking and simulation. The interplay between a cell cycle protein and a glycolytic enzyme highlights the connection between cellular metabolism and the cell cycle regulatory mechanism. The study serves as the groundwork for future research on the non-mitotic role of APC beyond cell cycle.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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