The herd-level prevalence of caprine arthritis-encephalitis and genetic characteristics of small ruminant lentivirus in the Lithuanian goat population.

Autor: Petkevičius S; Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes str. 18, Kaunas LT-47181, Lithuania. Electronic address: saulius.petkevicius@lsmu.lt., Klibavičė P; Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes str. 18, Kaunas LT-47181, Lithuania. Electronic address: patricija.klibavice@lsmu.lt., Šalomskas A; Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes str. 18, Kaunas LT-47181, Lithuania. Electronic address: algirdas.salomskas@lsmu.lt., Kupčinskas T; Department of Veterinary Pathobiology, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes str. 18, Kaunas LT-47181, Lithuania. Electronic address: tomas.kupcinskas@lsmu.lt., Moroz-Fik A; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: agata_moroz@sggw.edu.pl., Biernacka K; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: kinga_biernacka@sggw.edu.pl., Mickiewicz M; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: marcin_mickiewicz@sggw.edu.pl., Nowek Z; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: nowekz@wp.pl., Ózsvári L; Department of Veterinary Forensics and Economics, University of Veterinary Medicine Budapest, István u. 2, Budapest H-1078, Hungary. Electronic address: ozsvari.laszlo@univet.hu., Bárdos K; Department of Veterinary Forensics and Economics, University of Veterinary Medicine Budapest, István u. 2, Budapest H-1078, Hungary. Electronic address: bardos.krisztina@univet.hu., Stuen S; Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Svebastadveien 112, Sandnes N-4325, Norway. Electronic address: snorre.stuen@nmbu.no., Abril CE; Institute of Virology and Immunology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Laenggass-Str. 122, Bern CH-3012, Switzerland. Electronic address: carlos.abril-gaona@unibe.ch., Bertoni G; Institute of Virology and Immunology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Laenggass-Str. 122, Bern CH-3012, Switzerland. Electronic address: giuseppe.bertoni@unibe.ch., Kaba J; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: jaroslaw_kaba@sggw.edu.pl., Czopowicz M; Division of Veterinary Epidemiology and Economics, Institute of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159c, Warsaw 02-776, Poland. Electronic address: michal_czopowicz@sggw.edu.pl.
Jazyk: angličtina
Zdroj: Preventive veterinary medicine [Prev Vet Med] 2024 Oct 23; Vol. 233, pp. 106363. Date of Electronic Publication: 2024 Oct 23.
DOI: 10.1016/j.prevetmed.2024.106363
Abstrakt: Caprine arthritis-encephalitis (CAE) is a progressive disease of goats caused by small ruminant lentivirus (SRLV) and is considered as one of the most important threats for goat farming in developed countries. The disease prevalence has never been investigated in the Lithuanian goat population. Therefore, a descriptive cross-sectional study was carried out in 2021-2022 to determine if SRLV infection was present in the Lithuanian goat population and, in the case of a positive result, to estimate the true herd-level prevalence of SRLV infection and specify genotypes and subtypes of SRLV responsible for the infection. Thirty goat herds counting >5 adult goats were randomly selected and, in each herd, a representative sample of adult goats was blood-sampled and tested serologically for SRLV infection using a commercial ELISA. The herd was considered infected if at least one goat tested positive and the true herd-level prevalence of SRLV infection was estimated using the Bayesian approach. Seropositive animals were found in 17 / 30 herds (57 %; 95 % confidence interval: 39 %, 73 %). The true herd-level prevalence was 56 % (95 % credible interval: 36 %, 76 %). In 10 / 17 seropositive herds whose owners consented for resampling of seropositive goats, 1-5 seropositive goats were tested using the nested real-time PCR (nRT-PCR). Goats from 9 seropositive herds tested positive in the nRT-PCR: in 4 herds for genotype A, in 4 herds for genotype B, and in 1 herd - 2 goats for genotype B and 1 goat for genotype A. From each of 9 nRT-PCR-positive herds, 1 PCR product of each genotype was sequenced using Sanger method and the phylogenic tree was constructed using the neighbor-joining method in the Molecular Evolutionary Genetics Analysis software. Four herds turned out to be infected with B1 subtype (91 % identity with the prototypic strain), 3 herds with A2 subtype (90 %-92 % identity), and a herd with mixed infection was infected with B1 (91 % identity) and A2 subtype (90 % identity). In one herd, the only seropositive goat was found to be infected with the strain most closely related to the A1 subtype (80 % identity). This study shows for the first time that SRLV infection is present and widespread in the Lithuanian goat population and both classical SRLV genotypes, represented by quite typical subtypes A2 and B1, appear to be responsible for the infection.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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