Menthol as an effective inhibitor of quorum sensing and biofilm formation in Candida albicans and Candida glabrata by targeting the transcriptional repressor TUP1.

Autor: Khodavandi P; Department of Animal Science, Shiraz University, Shiraz, Iran., Soogh MM; Department of Microbiology, Yasuj Branch, Islamic Azad University, Yasuj, Iran., Alizadeh F; Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran., Khodavandi A; Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran. alireza_khodavandi@yahoo.com., Nouripour-Sisakht S; Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Nov 01; Vol. 51 (1), pp. 1114. Date of Electronic Publication: 2024 Nov 01.
DOI: 10.1007/s11033-024-10054-1
Abstrakt: Background: Menthol, a natural quorum sensing molecule, is derived from the Mentha species. Combating pathogenicity by inactivating quorum sensing is an emerging approach. Therefore, our objective was to investigate anti-quorum sensing and anti-biofilm potentials of menthol in Candida albicans and Candida glabrata.
Methods: The antifungal properties of menthol were evaluated using a broth microdilution assay and a time-kill assay, and its effects on quorum sensing-mediated virulence factors, cellular reactive oxygen species (ROS), and biofilm formation were tested by evaluating TUP1 expression levels in both C. albicans and C. glabrata.
Results: Quorum sensing-mediated virulence factors and biofilm formation were inhibited by menthol in both C. albicans and C. glabrata. Furthermore, coinciding with elevated ROS levels, mRNAs of the quorum sensing-related gene TUP1 were upregulated in both C. albicans and C. glabrata.
Conclusions: This study highlights the anti-quorum sensing potential of menthol through the inhibition of quorum sensing-mediated virulence factors, ROS generation, and biofilm development by targeting TUP1, which could have potential in the treatment of Candida infections.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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