Metallophosphoesterase-Domain-Containing Protein 2 (MPPED2) Expression in High-Risk Human Papilloma Virus-Induced Cervical Carcinoma and Its Correlation With p16INK4A Protein.

Autor:	Sujatha T; Pathology and Microbiology Division, Indian Council of Medical Research-National Institute of Nutrition, Hyderabad, IND., Jayashankar E; Pathology & Lab Medicine, All India Institute of Medical Sciences, Bhopal, Bhopal, IND., Uday Kumar P; Pathology and Microbiology Division, Indian Council of Medical Research-National Institute of Nutrition, Hyderabad, IND., Bhopal T; Department of Pathology, Mehdi Nawaz Jung Cancer Hospital, Hyderabad, IND., Manjunath R; Medical Records Unit, Chengalpattu Government Medical College, Chengalpattu, IND., Surekha MV; Pathology and Microbiology Division, Indian Council of Medical Research-National Institute of Nutrition, Hyderabad, IND.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Sep 30; Vol. 16 (9), pp. e70576. Date of Electronic Publication: 2024 Sep 30 (Print Publication: 2024).
DOI:	10.7759/cureus.70576
Abstrakt:	Introduction Recently, the expression of metallophosphoesterase-domain-containing protein 2 (MPPED2) was identified in cervical cancer. However, its precise role and correlation with other tumor suppressor proteins, such as p16INK4A, is not well studied in high-risk human papillomavirus (HPV) integrated human cervical carcinoma. Hence, in the present study, we try to see the expression of MPPED2 in human cervical carcinoma and its correlation with age and p16INK4A protein expression level. Methods The prospective study consists of 200 samples of 150 known cervical carcinoma and 50 controls. Histopathological evaluation, immunohistochemical staining, and semi-quantitative scoring of the intensity of proteins were performed. Statistical analysis was performed with the Shapiro-Wilk test, Spearman's rho correlation sig. (two-tailed), and Student's t-test. Results The data show that among the 150 cases, 136 (68.0%) cervical carcinoma tissues express the presence of high-risk HPV viral genome integration in the host cell. The expression of p16INK4A protein is higher in those tissues identified with high-risk HPV viral genomes. In contrast, the expression of MPPED2 protein is lesser or absent in those cervical tissues that have the higher expression of p16INK4A protein and vice versa. There is a significant correlation (p=0.000) between age and p16INK4A protein expression but not with MPPED2. A significant linear correlation (p=0.000) is found between the p16INK4A and MPPED2 proteins. Conclusion It may support the therapeutic application of MPPED2 protein to prevent cervical carcinoma progression in the near future. Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. MNJ Institute of Oncology and Regional Cancer Centre and National Institute of Nutrition, Hyderabad, India issued approval LS-359/2017. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: This study was funded by the Department of Science and Technology, India, through the Women Scientist A program. Dr. Sujatha Thathapudi awarded with Women Scientist A fellowship. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Sujatha et al.)
Databáze:	MEDLINE
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