The Effect of Doxapram on Proprioceptive Neurons: Invertebrate Model.

Autor:	Ison BJ; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Abul-Khoudoud MO; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Ahmed S; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Alhamdani AW; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Ashley C; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Bidros PC; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Bledsoe CO; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Bolton KE; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Capili JG; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Henning JN; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Moon M; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Phe P; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Stonecipher SB; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Tanner HN; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Turner LT; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Taylor IN; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Wagers ML; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., West AK; Department of Biology, University of Kentucky, Lexington, KY 40506, USA., Cooper RL; Department of Biology, University of Kentucky, Lexington, KY 40506, USA.
Jazyk:	angličtina
Zdroj:	NeuroSci [NeuroSci] 2022 Oct 23; Vol. 3 (4), pp. 566-588. Date of Electronic Publication: 2022 Oct 23 (Print Publication: 2022).
DOI:	10.3390/neurosci3040041
Abstrakt:	The resting membrane potential enables neurons to rapidly initiate and conduct electrical signals. K2p channels are key in maintaining this membrane potential and electrical excitability. They direct the resting membrane potential toward the K + equilibrium potential. Doxapram is a known blocker for a subset of K2p channels that are pH sensitive. We assessed the effects of 0.1 and 5 mM doxapram on the neural activity within the propodite-dactylopodite (PD) proprioceptive sensory organ in the walking legs of blue crabs ( Callinectes sapidus ). Results indicate that 0.1 mM doxapram enhances excitation, while the higher concentration 5 mM may over-excite the neurons and promote a sustained absolute refractory period until the compound is removed. The effect of 5 mM doxapram mimics the effect of 40 mM K + exposure. Verapamil, another known K2p channel blocker as well as an L-type Ca 2+ channel blocker, reduces neural activity at both 0.1 and 5 mM. Verapamil may block stretch activated channels in sensory endings, in addition to reducing the amplitude of the compound action potential with whole nerve preparations. These findings are notable as they demonstrate that doxapram has acute effects on neurons of crustaceans, suggesting a targeted K2p channel. The actions of verapamil are complex due to the potential of affecting multiple ion channels in this preparation. Crustacean neurons can aid in understanding the mechanisms of action of various pharmacological agents as more information is gained. Competing Interests: Conflicts of InterestThe authors declare that they have no conflict of interest. (© 2022 by the authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu