Deciphering craniopharyngioma subtypes: Single-cell analysis of tumor microenvironment and immune networks.

Autor:	Matsuda T; Department of Neurological Surgery Chiba University Graduate School of Medicine, Chiba, Japan.; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan., Kono T; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.; Research Institute of Disaster Medicine, Chiba University, Chiba, Japan., Taki Y; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan., Sakuma I; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan., Fujimoto M; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan., Hashimoto N; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.; Research Institute of Disaster Medicine, Chiba University, Chiba, Japan., Kawakami E; Department of Aritificial Intelligence Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan., Fukuhara N; Department of Hypothalamic and Pituitary Surgery, Toranomon Hospital, Tokyo, Japan., Nishioka H; Department of Hypothalamic and Pituitary Surgery, Toranomon Hospital, Tokyo, Japan., Inoshita N; Hypothalamic and Pituitary Center, Moriyama Memorial Hospital, Tokyo, Japan., Yamada S; Hypothalamic and Pituitary Center, Moriyama Memorial Hospital, Tokyo, Japan., Nakamura Y; Division of Pathology, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Miyagi, Japan., Horiguchi K; Department of Neurological Surgery Chiba University Graduate School of Medicine, Chiba, Japan., Miki T; Research Institute of Disaster Medicine, Chiba University, Chiba, Japan.; Department of Medical Physiology, Graduate School of Medicine, Chiba University, Chiba, Japan., Higuchi Y; Department of Neurological Surgery Chiba University Graduate School of Medicine, Chiba, Japan., Tanaka T; Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Chiba, Japan.; Research Institute of Disaster Medicine, Chiba University, Chiba, Japan.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2024 Oct 01; Vol. 27 (11), pp. 111068. Date of Electronic Publication: 2024 Oct 01 (Print Publication: 2024).
DOI:	10.1016/j.isci.2024.111068
Abstrakt:	Craniopharyngiomas, including adamantinomatous (ACP) and squamous papillary (PCP) types, are challenging to treat because of their proximity to crucial pituitary structures. This study aimed to characterize the cellular composition, tumor tissue diversity, and cell-cell interactions in ACPs and PCPs using single-cell RNA sequencing. Single-cell clustering revealed diverse cell types, further classified into developing epithelial, calcification, and immune response for ACP and developing epithelial, cell cycle, and immune response for PCP, based on gene expression patterns. Subclustering revealed the enrichment of classical M1 and M2 macrophages in ACP and PCP, respectively, with high expression of pro-inflammatory markers in classical M1 macrophages. The classical M1 and M2 macrophage ratio significantly correlated with the occurrence of diabetes insipidus and panhypopituitarism. Cell-cell interactions, particularly involving CD44-SPP, were identified between tumor cells. Thus, we developed a comprehensive cell atlas that elucidated the molecular characteristics and immune cell inter-networking in ACP and PCP tumor microenvironments. Competing Interests: The authors declare no competing interests. (© 2024 The Author(s).)
Databáze:	MEDLINE
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