A silver lining in MRSA treatment: The synergistic action of poloxamer-stabilized silver nanoparticles and methicillin against antimicrobial resistance.

Autor: Nijil S; Nitte (Deemed to be University), Department of Bio and Nano Technology, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, India., Bhat SG; Nitte (Deemed to be University), Department of Bio and Nano Technology, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, India., Kedla A; Nitte (Deemed to be University), Department of Bio and Nano Technology, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, India., Thomas MR; Nitte (Deemed to be University), Department of Bio and Nano Technology, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, India., Kini S; Nitte (Deemed to be University), Department of Bio and Nano Technology, Nitte University Centre for Science Education and Research, Deralakatte, Mangalore, 575018, India. Electronic address: sudarshan.kini@nitte.edu.in.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Dec; Vol. 197, pp. 107087. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1016/j.micpath.2024.107087
Abstrakt: Background: Increasing antibiotic resistance in bacterial infections, including drug-resistant strains like methicillin-resistant Staphylococcus aureus (MRSA), necessitates innovative therapeutic solutions. Silver nanoparticles are promising for combating infections, but toxicity concerns emphasize the importance of factors like dosage, size, shape, and surface chemistry. Hence, exploring poloxamer as a stabilizing agent to reduce its toxicity and enhance the antibacterial effect on MRSA is investigated.
Methods: Silver nanoparticles stabilized with poloxamer (AgNPs@Pol) were synthesized through the chemical reduction method and characterized using UV-visible spectrophotometer, HR-TEM, DLS, and Zeta potential measurements. Subsequently, the antibacterial activity of AgNPs@Pol alone and in combination with methicillin against MRSA and methicillin-susceptible S. aureus (MSSA) was evaluated using the broth microdilution method.
Results: AgNPs@Pol showed significant efficacy against MRSA and MSSA, achieving a 100 % reduction in colony-forming units (CFU) at 9.7 μg/ml. The minimum inhibitory concentration (MIC) against MRSA and MSSA was 8.6 μg/ml and 4.3 μg/ml, respectively. A synergistic effect was observed when AgNPs@Pol was combined with methicillin. Treatment with AgNPs@Pol increased reactive oxygen species (ROS) production in both strains, contributing to its antibacterial activity. Real-time qPCR analysis indicated the downregulation of genes involved in antimicrobial resistance and cell adhesion in both strains. Further, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay demonstrated low cytotoxicity for AgNPs@Pol against MCF-7, MG-63, and NIH-3T3 cell lines.
Conclusion: The developed AgNPs@Pol demonstrated extensive colloidal stability, potent antibacterial activity and synergistic effect with methicillin against MRSA and MSSA. Further studies in primary cells and in vivo models may validate its potential for clinical applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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