Genomic Diversity of HPV6 and HPV11 in Recurrent Respiratory Papillomatosis: Association with Malignant Transformation in the Lungs and Clinical Outcomes.

Autor:	Rahmoun M; Laboratory MIVEGEC (Univ Montpellier, CNRS, IRD), French National Center for Scientific Research (CNRS), Montpellier, France., Aussel A; Service d'anatomie et cytologie pathologiques, CHU de Montpellier, Montpellier, France., Bouzidi S; Laboratory MIVEGEC (Univ Montpellier, CNRS, IRD), French National Center for Scientific Research (CNRS), Montpellier, France., Pedergnana V; Laboratory MIVEGEC (Univ Montpellier, CNRS, IRD), French National Center for Scientific Research (CNRS), Montpellier, France., Malassigné V; Unité de Génomique Fonctionnelle des Tumeurs Solides, Centre de Recherche des Cordeliers, INSERM, Université Paris, Paris, France., Puech J; Laboratoire de Virologie, Service de Microbiologie, hôpital européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France., Veyer D; Unité de Génomique Fonctionnelle des Tumeurs Solides, Centre de Recherche des Cordeliers, INSERM, Université Paris, Paris, France; Laboratoire de Virologie, Service de Microbiologie, hôpital européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France., Péré H; Unité de Génomique Fonctionnelle des Tumeurs Solides, Centre de Recherche des Cordeliers, INSERM, Université Paris, Paris, France; Laboratoire de Virologie, Service de Microbiologie, hôpital européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France., Lepine C; Nantes University, CHU de Nantes, Pathology Department, Nantes, France; INSERM, CNRS, Immunology and New Concepts in ImmunoTherapy, INCIT, UMR 1302/EMR6001, Nantes, France., Blanc F; Department of Head and Neck Surgery, CHU de Montpellier, Montpellier, France., Boulle N; Pathogenesis and Control of Chronic and Emerging Infections, Department of Pathology and Oncobiology, Laboratory of Solid Tumors, CHU Montpellier, University of Montpellier, INSERM, EFS, Montpellier, France., Costes-Martineau V; Service d'anatomie et cytologie pathologiques, CHU de Montpellier, Montpellier, France., Bravo IG; Laboratory MIVEGEC (Univ Montpellier, CNRS, IRD), French National Center for Scientific Research (CNRS), Montpellier, France. Electronic address: ignacio.bravo@cnrs.fr.
Jazyk:	angličtina
Zdroj:	Tumour virus research [Tumour Virus Res] 2024 Oct 29, pp. 200294. Date of Electronic Publication: 2024 Oct 29.
DOI:	10.1016/j.tvr.2024.200294
Abstrakt:	Recurrent respiratory papillomatosis (RRP) is a rare, proliferative disease caused by human papillomavirus 6 (HPV6) and HPV11. RRP can occasionally spread and undergo malignant transformation. We analysed samples across time for five RRP patients with malignant transformation and four with highly recurrent, non-malignant RRP by applying high-throughput sequencing. Patients with malignant transformation were infected by HPV11_A1/A2, while most non-malignant cases were associated with HPV6. Transient multiple infections with HPV6 and HPV11 were found in two patients, and resolved later to single infections. Viral genome loads were homogeneous across groups (median=78 viral genomes per human genome). Within-patient, we did not observe differences between the viral sequences in the papillomatous lesions and in the malignant tissue. Genetic analysis of the NLRP1 gene revealed no known mutations linked to idiopathic RRP, though some novel variants merit to be explored in larger cohorts. HPV11 infections appear associated with RRP malignant transformation in young patients. Multiple infections can occur in RRP, but within-patient viral diversity is minimal for a given genotype. Our results confirm the importance of viral genotype in disease prognosis and are consistent with growing evidence of HPV11 infections to be differentially associated with RRP malignant transformation in young patients. Competing Interests: Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.)
Databáze:	MEDLINE
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