Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs.

Autor:	Baumann Z; Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; Department of Surgery, University Hospital Basel, 4031 Basel, Switzerland., Wiethe C; BioLegend, Inc., San Diego, CA 92121, USA., Vecchi CM; Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; Department of Surgery, University Hospital Basel, 4031 Basel, Switzerland., Richina V; Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; Department of Surgery, University Hospital Basel, 4031 Basel, Switzerland., Lopes T; Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland., Bentires-Alj M; Department of Biomedicine, University Hospital Basel, University of Basel, 4031 Basel, Switzerland; Department of Surgery, University Hospital Basel, 4031 Basel, Switzerland. Electronic address: m.bentires-alj@unibas.ch.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2024 Nov 18; Vol. 4 (11), pp. 100885. Date of Electronic Publication: 2024 Oct 30.
DOI:	10.1016/j.crmeth.2024.100885
Abstrakt:	The lung immune system consists of both resident and circulating immune cells that communicate intricately. The immune system is activated by exposure to bacteria and viruses, when cancer initiates in the lung (primary lung cancer), or when metastases of other cancer types, including breast cancer, spread to and develop in the lung (secondary lung cancer). Thus, in these pathological situations, a comprehensive and quantitative assessment of changes in the lung immune system is of paramount importance for understanding mechanisms of infectious diseases, lung cancer, and metastasis but also for developing efficacious treatments. Unfortunately, lung tissue exhibits high autofluorescence, and this high background signal makes high-parameter flow cytometry analysis complicated. Here, we provide an optimized 30-parameter antibody panel for the analysis of all major immune cell types and states in normal and metastatic murine lungs using spectral flow cytometry. Competing Interests: Declaration of interests C.W. is a current employee of BioLegend, Inc. (Copyright © 2024 University of Basel, Department of Biomedicine. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu