Novel förster resonance energy transfer (FRET)-based ratiometric fluorescent probe for detection of cyanides by nucleophilic substitution of aromatic hydrogen (SNArH).

Autor: She Q; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China., Cao Y; MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China., Zhou Y; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China., Tan Y; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China., Kan A; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China., Yang J; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China., Yan J; MOE International Joint Research Laboratory on Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: yjw@scut.edu.cn., Wu J; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China. Electronic address: wujj@sit.edu.cn., Liu C; School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China. Electronic address: cxliu@sit.edu.cn.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Feb 15; Vol. 327, pp. 125339. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1016/j.saa.2024.125339
Abstrakt: The development of novel fluorescent probes for real-time detection of cyanides (CN - ) in environmental and biological systems has become a significant focus in chemical sensing. Particularly, ratiometric fluorescence sensing offers a unique method for precise and quantitative detection of cyanides, even under complex conditions. We report herein the design of a new ratiometric fluorescent probe for cyanides based on modulation of Förster resonance energy transfer (FRET) coupled with novel cyanide-induced nucleophilic substitution of aromatic hydrogen (SNArH). The target probe (R1) is developed by introducing coumarin fluorophores as FRET donors into a 3-nitro-naphthalimide acceptor, which is easily synthesized and exhibits a colorimetric change from colorless to faint yellow and a significant ratiometric fluorescence shift (Δλ = 114 nm) upon cyanide binding. A clear ratiometric signal at I 582 /I 468 was obtained, with a limit of detection of 5.69 μM. The sensing mechanism was confirmed through 1 H NMR titration and LC-MS analysis. Additionally, R1-loaded strips were easily prepared, serving as a portable device for detecting CN - with visible color changes. The probe R1 has been successfully utilized for real-time monitoring of cyanide in food materials and water samples. Importantly, fluorescence bioimaging studies in HeLa cells were conducted, demonstrating the probe's capability for ratiometric detection of exogenous CN - in living systems.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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