Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer's Patches of the Rat Intestine.

Autor: Fedorova AA; Department of General Physiology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia., Rybalchenko OV; Department of Physiology, Institute of Medicine, St. Petersburg State University, St. Petersburg, Russia., Okorokova LS; Department of General Physiology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia., Kapustina VV; Department of Physiology, Institute of Medicine, St. Petersburg State University, St. Petersburg, Russia., Orlova OG; Department of Physiology, Institute of Medicine, St. Petersburg State University, St. Petersburg, Russia., Markov AG; Department of General Physiology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia. a.markov@spbu.ru.; Laboratory of Interoception, Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia. a.markov@spbu.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2024 Oct; Vol. 177 (6), pp. 757-762. Date of Electronic Publication: 2024 Oct 31.
DOI: 10.1007/s10517-024-06263-0
Abstrakt: To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer's patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types. LPS increased the number of small vesicles in follicle-associated epithelium of Peyers' patches. In columnar epithelial cells, LPS reduced the number of smaller vesicles and increased the number of larger ones. LPS did not damage the tissue barrier, but enhanced transcytosis, which could potentiate the effects of endotoxin on its receptors in the intestinal mucosa.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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