Molecular and Clinicopathologic Characterization of HER2-overexpressed Squamous Cell Carcinoma of the Cervix.

Autor:	Mendoza RP; Department of Pathology, University of Rochester Medical Center, Rochester., Ramineni M; Department of Pathology, University of Rochester Medical Center, Rochester., Doytcheva K; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Gabutan EC; Department of Pathology, State University of New York Downstate Health Sciences University, Brooklyn, New York., Gupta R; Department of Pathology, State University of New York Downstate Health Sciences University, Brooklyn, New York., Miller C; Department of Pathology, University of Rochester Medical Center, Rochester., Choi D; Department of Pathology, University of Rochester Medical Center, Rochester., Vemuri A; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Briese R; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Brannon L; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Shahid A; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Petras K; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Ud Dean M; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Fitzpatrick C; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Segal J; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Wang P; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois., Lastra RR; Department of Pathology, University of Chicago Medical Center, Chicago, Illinois.
Jazyk:	angličtina
Zdroj:	International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists [Int J Gynecol Pathol] 2024 Oct 31. Date of Electronic Publication: 2024 Oct 31.
DOI:	10.1097/PGP.0000000000001068
Abstrakt:	HER2 amplification in cervical cancer has been associated with worse clinical prognosis and a potential favorable response to HER2 inhibitors. Immunohistochemistry for the HER2 receptor is a universally accepted surrogate test for HER2 amplification, but no standardized scoring system currently exists for cervical carcinomas. In this study, we investigated HER2 overexpression in cervical squamous cell carcinoma and correlated it with HER2 amplification using fluorescence in situ hybridization (FISH) and molecular methods. Seventy-two cases of human papillomavirus-associated cervical cancer were retrospectively reviewed, and at least 2 representative tumor sections were stained for HER2. HER2 scoring was performed using the 2018 American Society of Clinical Oncology/College of American Pathologist breast cancer criteria, and cases with equivocal (2+) to positive (3+) expression were analyzed for HER2 amplification using FISH and next-generation sequencing. The average patient age was 50 yrs (range: 27-85 yr), with most patients being African American (73.6%) and diagnosed at FIGO stage I (65.3%). Nineteen (26.4%) had equivocal HER2 expression and 4 (5.5%) showed positive expression. Three of the 4 cases with positive expression had enough tumors for FISH, and all 3 were amplified. Three cases with equivocal expression showed HER2 polysomy on FISH, and none showed HER2 amplification. Late clinical stage, high tumor grade, and regional lymph node metastasis were significantly correlated with HER2 overexpression and HER2 amplification. Next-generation sequencing of the 3 HER2-amplified tumors showed amplification of various genes, including CD274, JAK2, BIRC3, and ERBB2, and a PIK3CA missense mutation. In summary, HER2 immunohistochemistry is a reliable predictive marker of HER2 amplification in cervical cancer. Competing Interests: The authors declare no conflict of interest. (Copyright © 2024 by the International Society of Gynecological Pathologists.)
Databáze:	MEDLINE
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