[Analysis of RhC Antigen Weak Expression Combined with Mimicking Autoanti-Ce and Homologous Anti-Jkb Causing Mismatch].

Autor:	Yang HM; Changzhou Blood Center, Changzhou 213000, Jiangsu Province, China.; Changzhou Key Laboratory of Clinical Blood Transfusion, Changzhou 213000, Jiangsu Provine, China., Yu X; Changzhou Blood Center, Changzhou 213000, Jiangsu Province, China.; Changzhou Key Laboratory of Clinical Blood Transfusion, Changzhou 213000, Jiangsu Provine, China., Zou X; Changzhou Blood Center, Changzhou 213000, Jiangsu Province, China.; Changzhou Key Laboratory of Clinical Blood Transfusion, Changzhou 213000, Jiangsu Provine, China., Ma SF; Changzhou Blood Center, Changzhou 213000, Jiangsu Province, China.; Changzhou Key Laboratory of Clinical Blood Transfusion, Changzhou 213000, Jiangsu Provine, China., Chen J; Department of Blood Transfusion, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou 213003, Jiangsu Province, China., Zhang JW; Changzhou Blood Center, Changzhou 213000, Jiangsu Province, China.; Changzhou Key Laboratory of Clinical Blood Transfusion, Changzhou 213000, Jiangsu Provine, China.
Jazyk:	čínština
Zdroj:	Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2024 Oct; Vol. 32 (5), pp. 1539-1544.
DOI:	10.19746/j.cnki.issn.1009-2137.2024.05.036
Abstrakt:	Objective: To investigate the reasons for the weak expression of RHCE gene in a patient whose mimicking anti-Ce combined with anti-Jkb caused cross-matching non-combination. Methods: ABO, Rh, and Kidd blood group antigens were identified by test tube method and capillary centrifugation. Antibody screening and antibody specificity identification were performed using saline, polybrene and antiglobulin in tri-media association with multispectral cells. RHCE gene sequencing and haploid analysis were performed by multiplex PCR technique and RHCE protein modeling was performed using Swiss-Model. Results: The serum of the patient contained anti-Ce mimicking autoantibodies along with anti-Jkb antibodies. c.48G>C, c.150C>T, c.178C>A, c.201A>G, c.203A>G, and c.307C>T mutations were detected in the RHCE triple-molecule sequencing. A 109 bp insertion sequence was found in intron 2, with fragment loss from intron 5-8. The Rh-group genotype was DCe/DCe , and phenotype was CCDee. Conclusion: Genotyping techniques can assist in deducing the molecular mechanisms of some weakly expressed RhC, c, E, and e in patients' sera to aid in the identification of difficult antibodies and thus ensure the safety of patients' blood transfusion.
Databáze:	MEDLINE
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