The replicative helicase CMG is required for the divergence of cell fates during asymmetric cell division in vivo.

Autor: Memar N; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK. nmemar@ibs.re.kr.; Center for Genomic Integrity, Institute for Basic Science (IBS), Ulsan, South Korea. nmemar@ibs.re.kr., Sherrard R; Faculty of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, Germany., Sethi A; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK., Fernandez CL; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK., Schmidt H; Institute of Genetics, TU Braunschweig, Braunschweig, Germany., Lambie EJ; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK., Poole RJ; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK., Schnabel R; Institute of Genetics, TU Braunschweig, Braunschweig, Germany., Conradt B; Research Department Cell and Developmental Biology, Division of Biosciences, University College London, London, UK. b.conradt@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Oct 30; Vol. 15 (1), pp. 9399. Date of Electronic Publication: 2024 Oct 30.
DOI: 10.1038/s41467-024-53715-2
Abstrakt: We report that the eukaryotic replicative helicase CMG (Cdc45-MCM-GINS) is required for differential gene expression in cells produced by asymmetric cell divisions in C. elegans. We found that the C. elegans CMG component, PSF-2 GINS2, is necessary for transcriptional upregulation of the pro-apoptotic gene egl-1 BH3-only that occurs in cells programmed to die after they are produced through asymmetric cell divisions. We propose that CMG's histone chaperone activity causes epigenetic changes at the egl-1 locus during replication in mother cells, and that these changes are required for egl-1 upregulation in cells programmed to die. We find that PSF-2 is also required for the divergence of other cell fates during C. elegans development, suggesting that this function is not unique to egl-1 expression. Our work uncovers an unexpected role of CMG in cell fate decisions and an intrinsic mechanism for gene expression plasticity in the context of asymmetric cell division.
(© 2024. The Author(s).)
Databáze: MEDLINE
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