RNAi-mediated silencing of transferrin promotes entomopathogens lethality in Odontotermes formosanus (Shiraki).

Autor: Wang Z; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China., Zhou Y; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China., Tang F; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China. Electronic address: tangfang76@sohu.com.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Nov; Vol. 205, pp. 106149. Date of Electronic Publication: 2024 Sep 24.
DOI: 10.1016/j.pestbp.2024.106149
Abstrakt: Transferrin (Tsf) is a highly conserved multifunctional protein involved in insect physiology, defense and development that has been developed as a novel RNA interference (RNAi)-based target for pest control. The function study of the Tsf gene in Odontotermes formosanu (Shiraki) was evaluated for synergistic control of this agroforestry pest with Serratia marcescens (SM1), Bacillus thuringiensis (Bt) or Beauveria bassiana (Bb). The Tsf gene of O. formosanus was identified and characterized. Real-time fluorescent quantitative PCR (qPCR) analysis demonstrated that OfTsf was most highly expressed in the male dealate of O. formosanus, and OfTsf was highly expressed in the hemolymph. OfTsf expression was considerably elevated after SM1, Bt or Bb infection. Furthermore, dsOfTsf treatment was effective in increasing the virulence of entomopathogens to O. formosanus. In addition, OfTsf expression was markedly upregulated in O. formosanus fed with oxidative stress inducers; reactive oxygen species (ROS) levels were significantly increased after dsOfTsf treatment. Therefore, OfTsf gene played an important role in defending against entomopathogen infection and antioxidant stress. Most importantly, our work suggested OfTsf as a potential RNAi target for the control of O. formosanus.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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