The genetic composition of Anopheles mosquitoes and the diverse population of gut-microbiota within the Anopheles subpictus and Anopheles vagus mosquitoes in Tamil Nadu, India.

Autor: Vinayagam S; Department of Biotechnology, Periyar University Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu 635 205, India., Sekar K; Department of Biotechnology, Periyar University Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu 635 205, India., Rajendran D; Department of Biotechnology, Periyar University Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu 635 205, India., Meenakshisundaram K; The University of Trans-Disciplinary, Bengaluru, Karnataka 560 064, India., Panigrahi A; Khallikote Unitary University, Ganjam, Odisha 760 008, India., Arumugam DK; Department of Biotechnology, Periyar University Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu 635 205, India., Bhowmick IP; ICMR-Regional Medical Research Centre North East Region, Dibrugarh, Assam 786010, India., Sattu K; Department of Biotechnology, Periyar University Centre for Postgraduate and Research Studies, Dharmapuri, Tamil Nadu 635 205, India. Electronic address: kamaraj07@periyaruniversity.ac.in.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Dec; Vol. 260, pp. 107439. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1016/j.actatropica.2024.107439
Abstrakt: In recent days, in tropical and subtropical regions, secondary vectors of Anopheles mosquitoes are becoming more important in transmitting diseases to humans as primary vectors. Various molecular techniques have separated closely related Anopheles subpictus and Anopheles vagus mosquitoes based on their diversity with other mosquito species. Despite their widespread distribution, the An. subpictus and An. vagus mosquitoes, which carry Plasmodium in their salivary glands, were not considered primary malaria vectors in India. An. vagus mosquitoes are zoophilic and physically similar to An. subpictus. We intend to identify An. subpictus and An. vagus mosquito's sister species based on their Interspaced Transcribed Region-2 (ITS2). We isolated the midgut gDNA from each mosquito and used ITS2-PCR and Sanger sequencing to characterize the mosquito species. BioEdit software aligned the sequences, and MEGA7 built a phylogenetic tree from them. According to this study, the information gathered from these mosquito samples fits the An. subpictus species A form and the An. vagus Indian form. Furthermore, gut microbiome plays an important role in providing nutrients, immunity, and food processing, whereas mosquitoes' midgut microbiota changes their hosts and spreads illnesses. So, we used the Illumina sequencer to look at the gut microbiome diversity of An. subpictus and An. vagus mosquitoes using 16S rRNA-based metagenomic sequencing. Both mosquito species had an abundant phylum of Pseudomonadota (Proteobacteria), Bacillota, Bacteroidota, and Actinomycetota in their gut microbiomes. Notably, both mosquito species had the genus Serratia in their gut. In the subpictus midgut, the genus of Haematosprillum bacteria was dominant, whereas in the vagus mosquito, the genus of Salmonella was dominant. Notably, current research has observed the Sodalis spp. Bacterial genus for the first time.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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