In vivo evaluation of anti-diarrheal activity of hydroalcoholic extract and solvent fractions of the leaf of Leucas deflexa Hook.f (Lamiaceae) in rodents.

Autor: Hammeso WW; Department of Pharmacy, College of Medicine and Health Sciences, Dilla University, Dilla, Ethiopia. Electronic address: workinehws@yahoo.com., Fisseha N; School of Pharmacy, Department Pharmacology, College of Medicine and Health Sciences, Mizan-Tepi University, Mizan-Aman, Ethiopia., Abera T; School of Pharmacy, Department Pharmacology, College of Medicine and Health Sciences, Mizan-Tepi University, Mizan-Aman, Ethiopia., Nureye D; School of Pharmacy, Department Pharmacology, College of Medicine and Health Sciences, Mizan-Tepi University, Mizan-Aman, Ethiopia., Woldeselassie BD; Department of Statistics, College of Natural and Computational Sciences, Dilla University, Dilla, Ethiopia.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2025 Feb 10; Vol. 338 (Pt 1), pp. 118993. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1016/j.jep.2024.118993
Abstrakt: Ethnopharmacological Relevance: The emergence of antibiotic-resistant bacteria has intensified the search for novel antidiarrheal drug. Plant-derived extracts offer promising alternatives due to their ability to modulate gut motility and enhance water absorption. The leaves of Leucas deflexa Hook.f., a plant native to Ethiopia, have been traditionally utilized in the treatment of diarrheal diseases. Nonetheless, its traditional application has yet to be scientifically confirmed.
Aim of the Study: Hence, the aim of the present study was to evaluate antidiarrheal activity of the crude extract and fractions of the leaf of Leucas deflexa Hook.f (Lamiaceae).
Materials and Methods: Healthy Swiss albino mice were randomly divided into five groups per experimental model: castor oil-induced diarrhea, gastrointestinal motility, and anti-enteropooling assays. The control group received distilled water (10 ml/kg), while the positive control was treated with loperamide (3 mg/kg). The remaining groups were administered various dosages (LD100, LD200, and LD400 mg/kg) of a hydroalcoholic extract or its fractions from Leucas deflexa Hook.f.
Result: The crude extract and chloroform fraction substantially (P < 0.001) delayed the onset of diarrhea, reduced fecal frequency, and decreased total fecal weight at all administered doses compared to the negative control. The butanol and aqueous fractions exhibited a substantial antidiarrheal effect at doses of 200 mg/kg and 400 mg/kg, respectively. All treatments, including the crude extract, chloroform, butanol, and aqueous fractions, substantially (P < 0.001) reduced the volume and weight of intestinal contents at various doses. Additionally, the chloroform, aqueous fraction, and crude extract at doses of 100, 200, and 400 mg/kg substantially inhibited gastrointestinal motility compared to the negative control.
Conclusion: The results of this study corroborate the traditional use of Leucas deflexa leaves in the treatment of diarrhea. These findings give a scientific justification for further exploration of the underlying mechanisms and potential therapeutic applications of this plant extract.
Competing Interests: Declaration of competing interest We have no conflicts of interest to disclose associated with this publication This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors that could have influenced its outcome. We understand that the Corresponding Author is the sole contact for the Editorial process. He is responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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