The association of combined GSTM1, GSTT1, and GSTP1 genetic polymorphisms with lung cancer risk in male Iraqi Waterpipe Tobacco (Nargila) smokers.

Autor: Kudhair BK; Department of Laboratory Investigations, Faculty of Science, University of Kufa, Najaf, Iraq. Electronic address: bassamk.sharuza@uokufa.edu.iq., Abdulridha FM; Department of Medical Laboratory Techniques, Faculty of Medical Technology and Health, University of Alkafeel, Najaf, Iraq., Hussain GM; Department of Medical Laboratory Techniques, Faculty of Medical Technology and Health, University of Alkafeel, Najaf, Iraq., Lafta IJ; Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq., Alabid NN; Faculty of Education, University of Kufa, Najaf, Iraq.
Jazyk: angličtina
Zdroj: Cancer epidemiology [Cancer Epidemiol] 2024 Dec; Vol. 93, pp. 102689. Date of Electronic Publication: 2024 Oct 30.
DOI: 10.1016/j.canep.2024.102689
Abstrakt: Mutations in genes encoding proteins necessary for detoxifying oxidative stress products have been predicted to increase susceptibility to lung cancer (LC). Despite this, the association between waterpipe tobacco smoking (WP), genetic polymorphisms, and LC risk remains poorly understood. This is the first study to explore the relationship between WP tobacco smoking and these genetic factors. Previously, we investigated the association of GSTP1 SNPs (rs1695-A/G and rs1138272-C/T) with LC in Iraqi males who smoke WP. Here, we expanded our analysis to include GSTM1 (active/null) and GSTT1 (active/null) genotypes, both individually and in combination with GSTP1 SNPs. Multiplex PCR and RFLP-PCR assays were utilized to determine the genotypes of 123 cases and 129 controls. No significant association was observed between GSTM1-null or GSTT1-null genotypes and LC risk, either separately or in combination with variant genotypes of GSTP1 (rs1695 "AG+GG" and rs1138272 "CT+TT"). However, smoking WP and carrying null genotypes elevated the risk five-fold for GSTM1-null (OR 5.17, 95 % CI 2.02-13.24, P<0.001) and three-fold for GSTT1-null (OR 3.08, 95 % CI 1.55-6.13, P=0.001) compared to non-smokers carrying active genotypes. Conversely, genotype distribution analysis based on LC histological types did not indicate an increased risk of LC. Lung cancer is a complex multifactorial disease. WP smoking and GSTs genetic polymorphisms might be associated with an increased risk of developing LC. However, our data did not confirm an association between GST polymorphisms alone and the risk of LC.
Competing Interests: Declaration of Competing Interest None of the authors have any non-financial conflict of interest. The authors also declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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