Two-color fluorescence-guided surgery for head and neck cancer resections.

Autor:	Szafran DA; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States., Shams NA; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States., Montaño A; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States., Rizvi SZH; Oregon State University, Department of Pharmaceutical Science, College of Pharmacy, Portland, Oregon, United States., Alani AWG; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States.; Oregon State University, Department of Pharmaceutical Science, College of Pharmacy, Portland, Oregon, United States., Samkoe KS; Dartmouth College, Thayer School of Engineering, Hanover, New Hampshire, United States.; Dartmouth College, Geisel School of Medicine, Hanover, New Hampshire, United States., Wang LG; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States.; Oregon Health & Science University, Knight Cancer Institute, Portland, Oregon, United States., Gibbs SL; Oregon Health & Science University, Biomedical Engineering Department, Portland, Oregon, United States.; Oregon Health & Science University, Knight Cancer Institute, Portland, Oregon, United States.
Jazyk:	angličtina
Zdroj:	Journal of biomedical optics [J Biomed Opt] 2025 Jan; Vol. 30 (Suppl 1), pp. S13707. Date of Electronic Publication: 2024 Oct 29.
DOI:	10.1117/1.JBO.30.S1.S13707
Abstrakt:	Significance: Head and neck squamous cell carcinoma (HNSCC) has the sixth highest incidence worldwide, with > 650,000 cases annually. Surgery is the primary treatment option for HNSCC, during which surgeons balance two main goals: (1) complete cancer resection and (2) preservation of normal tissues to ensure post-surgical quality of life. Unfortunately, these goals are not synergistic, where complete cancer resection is often limited by efforts to preserve normal tissues, particularly nerves, and reduce life-altering comorbidities. Aim: Currently, no clinically validated technology exists to enhance intraoperative cancer and nerve recognition. Fluorescence-guided surgery (FGS) has successfully integrated into clinical medicine, providing surgeons with real-time visualization of important tissues and complex anatomy, where FGS imaging systems operate almost exclusively in the near-infrared (NIR, 650 to 900 nm). Notably, this spectral range permits the detection of two NIR imaging channels for spectrally distinct detection. Approach: Herein, we evaluated the utility of spectrally distinct NIR nerve- and tumor-specific fluorophores for two-color FGS to guide HNSCC surgery. Using a human HNSCC xenograft murine model, we demonstrated that facial nerves and tumors could be readily differentiated using these nerve- and tumor-specific NIR fluorophores. Results: The selected nerve-specific fluorophore showed no significant difference in nerve specificity and off-target tissue fluorescence in the presence of xenograft head and neck tumors. Co-administration of two NIR fluorophores demonstrated successful tissue-specific labeling of nerves and tumors in spectrally distinct NIR imaging channels. Conclusions: We demonstrate a comprehensive FGS tool for cancer resection and nerve sparing during HNSCC procedures for future clinical translation. (© 2024 The Authors.)
Databáze:	MEDLINE
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