Soluble Guanylate Cyclase Dysfunction and Nitric Oxide Pathway in Chronic Rhinosinusitis With Nasal Polyps: Predictive Markers for Postoperative Recurrence.

Autor:	Jiang Md S; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China., Zhang Md C; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China., Tong Md Z; Department of Otolaryngology Head and Neck Surgery, The First People's Hospital of Changde, Changde, China., Xie PhD S; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China., Xie Md Z; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China., Zhang Md H; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China., Jiang Md W; Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, China.; Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, China.; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, China.; Anatomy Laboratory of Division of Nose and Cranial Base, Clinical Anatomy Center of Xiangya Hospital, Central South University, Changsha, China.
Jazyk:	angličtina
Zdroj:	American journal of rhinology & allergy [Am J Rhinol Allergy] 2025 Jan; Vol. 39 (1), pp. 63-75. Date of Electronic Publication: 2024 Oct 30.
DOI:	10.1177/19458924241289645
Abstrakt:	Background: Elevated nitric oxide (NO) levels have been linked to a heightened risk of recurrence in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). However, the precise influence of NO on CRSwNP recurrence remains unclear. Objective: This study seeks to elucidate the relationship between NO levels and the risk of CRSwNP recurrence. Methods: A protein chip array analysis was conducted to identify differentially expressed inflammatory mediators in the nasal tissues between patients with CRSwNP and healthy controls (HC). Differentially expressed proteins were analyzed, and bioinformatics analysis was used to predict the potential functions and pathways of these proteins. Western blotting (WB) and immunohistochemistry were employed to validate the candidate proteins in 2 independent cohorts. Receiver-operating characteristic (ROC) curves were employed to assess the abilities of target proteins for predicting the postoperative recurrence of CRSwNP. Results: Twelve differentially expressed proteins were identified between the CRSwNP and HC groups. Notably, differentially expressed proteins exhibited high expression of the biological process term "positive regulation of nitric oxide-mediated signal transduction" ( P  < .05). WB and immunohistochemistry results demonstrated that guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), GUCY1A2, nitric oxide synthase 1 adaptor protein, epidermal growth factor receptor, and insulin were found to be upregulated in the CRSwNP group compared to the HC group ( P  < .05). Moreover, elevated levels of GUCY1A2 and GUCY1A1 were observed to be associated with an increased risk of CRSwNP recurrence ( P  < .05), and ROC curve analysis confirmed their effectiveness as predictors for postoperative recurrence ( P  < .05). Conclusion: Our findings revealed that CRSwNP exhibited a distinct tissue protein profile, with soluble guanylate cyclase dysfunction and the nitric oxide pathway implicated in the underlying pathological mechanisms. The discovery-validation results suggested that GUCY1A1 and GUCY1A2 were promising predictors for postoperative recurrence in patients with CRSwNP. Competing Interests: Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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