Enhancing impact of dietary nano formulated quercetin on laying performance: egg quality, oxidative stability of stored eggs, intestinal immune and antioxidants related genes expression.

Autor: Al-Khalaifah HS; Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box:24885, Safat, 13109, Kuwait., Ibrahim D; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44511, Egypt., Kamel AE; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44511, Egypt., Al-Nasser A; Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box:24885, Safat, 13109, Kuwait., Abdelwarith AA; Department of Zoology, College of Science, King Saudi University, Riyadh, 11451, Saudi Arabia., Roushdy EM; Department of Animal Wealth Development, Animal Breeding, and Production, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44511, Egypt., Sheraiba NI; Department of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, University of Sadat City, Sadat city, 32897, Egypt., Shafik BM; Department of Animal Wealth Development, Animal and Poultry Production, Faculty of Veterinary Medicine, Benha University, P.O. Box 13736, Toukh city, Qalyubia, Egypt., El-Badry SM; Department of Animal Wealth Development, Veterinary Genetics & Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44519, Egypt., Younis EM; Department of Zoology, College of Science, King Saudi University, Riyadh, 11451, Saudi Arabia., Mamdouh M; Department of Physiology, Faculty of Veterinary Medicine, Benha University, P.O. Box 13736, Toukh city, Qalyubia, Egypt., Yassin EMM; Department of Biochemistry and molecular biology, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44511, Egypt., Davies SJ; Aquaculture Nutrition Research Unit (ANRU), Carna Research Station, College of Science and Engineering, Ryan Institute, University of Galway, Galway, Ireland., Kishawy ATY; Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, 44511, Egypt. no.dispair2000@gmail.com.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Oct 29; Vol. 20 (1), pp. 494. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1186/s12917-024-04327-x
Abstrakt: Background: Nutritional interventions with natural antioxidants can provide a pragmatic solution for modifying hens' performance and maintaining oxidative stability of eggs during storage. Quercetin is the most abundant flavonoids with potent antioxidant and immune stimulant activities. The concept of incorporating of quercetin, as potent antioxidant and immunostimulant, into effective nano-carriers (QNPs) has promoted their bioavailability and stability thus, their effectiveness for the first time were assessed on laying hens' performance and immunity, eggs quality during storage. Four hundred 12-weeks-old Hy-line brown laying hens were distributed to four experimental groups: control group fed basal diets, and other 3 groups fed basal diets fortified with 100, 200 and 300 mg/kg QNPs for 60 weeks.
Results: Laying performance and quality of laid eggs were improved as expressed by elevated laying rate, egg mass %, eggs weight and yolk weight in QNPs200 and 300. Fortification of QNPs300 remarkably decreased layers serum total cholesterol concurrently with decreased egg yolk saturated fatty acids and cholesterol while increased polyunsaturated fatty acids. Over- 45 days storage period, QNPs enhanced phospholipids, total phenolics and flavonoids, total antioxidant activity (T-AOC) simultaneous with decreased MDA content in eggs. Furthermore, enhanced immune response was detected in both in serum and intestine of QNPs fed hens as reflected by higher lysozymes activity, IgM, IgG and phagocytic index and demotion of NO together with AvBD 6-12, IL-10, IgM and ATg 5-7-12 upregulation and downregulation of IL-1β and TNF-α especially at QNPs200 and 300. Intestinal redox balance was modified via decreasing H 2 O 2 and MDA simultaneous with upregulation of catalase, SOD, GSH-Px, HO-1 and NQO1 in groups fed higher doses of QNPs.
Conclusions: QNPs supplementation provides a new nutritional strategy towards increasing hen performance, fortification of eggs with natural antioxidants that prevents egg quality deterioration during storage.
(© 2024. The Author(s).)
Databáze: MEDLINE
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