Alteration of the metabolite interconversion enzyme in sperm and Sertoli cell of non-obstructive azoospermia: a microarray data and in-silico analysis.

Autor: Hashemi Karoii D; Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.; Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran., Baghaei H; Pediatric Urology and Regenerative Medicine Research Center, Gene, Cell and Tissue Research Institute, Children's Medical Center, Tehran University of Medical University, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, Tehran, 1419733151, Iran., Abroudi AS; Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran., Djamali M; Department of Biology, Faculty of Science, Tehran University, Tehran, Iran., Hasani Mahforoozmahalleh Z; Department of Microbial Biotechnology, Amol University of Special Modern Technologies, Amol, Iran., Azizi H; Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran. H.azizi@ausmt.ac.ir., Skutella T; Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Im Neuenheimer Feld 307, 69120, Heidelberg, Germany.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 29; Vol. 14 (1), pp. 25965. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1038/s41598-024-77875-9
Abstrakt: Numerous variables that regulate the metabolism of Sertoli cells and sperm have been identified, one of which is sex steroid hormones. These hormones play a vital role in maintaining energy homeostasis, influencing the overall metabolic balance of the human body. The proper functioning of the reproductive system is closely linked to energy status, as the reproductive axis responds to metabolic signals. The aim of this study was to investigate the gene expression patterns of metabolite interconversion enzymes in testicular cells (Sertoli cells and spermatogonia) of non-obstructive azoospermia (NOA) patients, as compared to normal controls, to understand the molecular mechanisms contributing to NOA. We used microarray and bioinformatics techniques to analyze 2912 genes encoding metabolite interconversion enzymes, including methyltransferase, monooxygenase, transmembrane reductase, and phosphohydrolase, in both testicular cells and normal samples. In sperm, the upregulation of MOXD1, ACAD10, PCYT1A, ARG1, METTL6, GPLD1, MAOA, and CYP46A1 was observed, while ENTPD2, CPT1C, ADC, and CYB5B were downregulated. Similarly, in the Sertoli cells of three NOA patients, RPIA, PIK3C3, LYPLA2, CA11, MBOAT7, and HDHD2 were upregulated, while NAA25, MAN2A1, CYB561, PNPLA5, RRM2, and other genes were downregulated. Using STRING and Cytoscape, we predicted the functional and molecular interactions of these proteins and identified key hub genes. Pathway enrichment analysis highlighted significant roles for G1/S-specific transcription, pyruvate metabolism, and citric acid metabolism in sperm, and the p53 signaling pathway and folate metabolism in Sertoli cells. Additionally, Weighted Gene Co-expression Network Analysis (WGCNA) and single-cell RNA sequencing (scRNA-seq) were performed to validate these findings, revealing significant alterations in gene expression and cellular distribution in NOA patients. Together, these results provide new insights into the molecular mechanisms underlying NOA and identify potential therapeutic targets.
(© 2024. The Author(s).)
Databáze: MEDLINE
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