Development of novel FRET aptasensor based on the quenching ability of iron oxide-gold nanostars for the detection of aflatoxin M1.

Autor: Haq FU; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China., Batool A; National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan., Niazi S; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China., Khan IM; Nottingham Ningbo China Beacons of Excellence Research and Innovation Institute, University of Nottingham Ningbo China, Ningbo 315100, China. Electronic address: imran-mahmood.khan@nottingham.edu.cn., Raza A; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China., Ning D; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China., Zhang Y; Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China., Wang Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Quality Control of Jiangsu Province, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Feb 01; Vol. 464 (Pt 2), pp. 141575. Date of Electronic Publication: 2024 Oct 16.
DOI: 10.1016/j.foodchem.2024.141575
Abstrakt: Detecting Aflatoxin M1 (AFM1) in food products is crucial due to its high toxicity and health risks. This study introduced a fluorescence donor material using Rhodamine-B-Isothiocyanate (RBITC)-doped silica nanoparticles (RDSN) combined with iron oxide‑gold nanostars (IOGNS) as a quencher. The composite aptasensor (RDSN/IOGNS) served as a Förster Resonance Energy Transfer (FRET) nanoprobe for sensitive and selective AFM1 detection. The fluorescence of aptamer-conjugated RDSN (apt-RDSN) was effectively quenched by complementary DNA-conjugated IOGNS (cDNA-IOGNS). Upon AFM1 introduction, apt-RDSN dissociated from the IOGNS surface, restoring the fluorescence signal. The fluorescence intensity correlated linearly with AFM1 concentration, achieving a detection limit of 0.15 ng/mL. Compared to conventional enzyme-linked immunosorbent assay (ELISA), this FRET aptasensor showed excellent recovery rate and relative standard deviation (RSD) in milk samples, highlighting its potential for practical AFM1 detection applications.
Competing Interests: Declaration of competing interest We declare that there are no financial interests or personal relationships that could have impacted the research presented in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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