Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata.

Autor: Al-Hasabe ASH; Department of Cell & Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia.; Department of Biology, Faculty of Science, Mustansiriyah University, Baghdad, Iraq., Abdull Razis AFB; Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia., Baharum NAB; Department of Cell & Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia., Yu CY; Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia., Mat Isa N; Department of Cell & Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia. nurulfiza@upm.edu.my.; Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia. nurulfiza@upm.edu.my.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2024 Oct 29; Vol. 206 (11), pp. 447. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1007/s00203-024-04182-2
Abstrakt: Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using Enterobacter chuandaensis with fruit extracts from Phoenix dactylifera (D) and Musa acuminata (M) as carbon sources. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) showed characteristic cellulose vibrations, while X-ray diffraction (XRD) identified distinct peaks at 15.34°, 19.98°, 22.58°, and 34.6°, confirming the cellulose structure. Whole-genome sequencing of E. chuandaensis identified key genes involved in BC production. The BC produced then exhibited a molecular weight of 1,857,804 g/mol, with yields of 2.8 g/L and 2.5 g/L for treatments D and M, respectively. The crystallinity index of the purified BC was 74.1, and 13 C NMR analysis confirmed the dominant cellulose Iα crystalline form. The BC showed high biocompatibility in cytotoxicity assays, with cell viability between 92% and 100%, indicating its potential for use in biomedical applications. This investigation represents the first report of BC production by E. chuandaensis, which promises a new avenue for sustainable and efficient BC synthesis using fruit extracts as carbon sources.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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