Biocompatible Zn-Phthalocyanine/Gelatin Nanofiber Membrane for Antibacterial Therapy.

Autor: Clementi R; Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina., Vargas MA; Laboratorio de Microbiología, Hospital Provincial Florencio Diaz, Córdoba, Argentina., Cid M; Facultad Ciencias Exactas, Físicas y Naturales, Departamento de Química, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 1611, Córdoba, 5016, Argentina.; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT), Córdoba, Argentina., Salvatierra N; Facultad Ciencias Exactas, Físicas y Naturales, Departamento de Química, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 1611, Córdoba, 5016, Argentina.; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT), Córdoba, Argentina., Comín R; Facultad Ciencias Exactas, Físicas y Naturales, Departamento de Química, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 1611, Córdoba, 5016, Argentina.; CONICET, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT), Córdoba, Argentina., Tempesti T; Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina.; INFIQC-CONICET, Instituto de Investigaciones en Físico-Química de Córdoba, Córdoba, Argentina.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2024 Oct 29, pp. e2400334. Date of Electronic Publication: 2024 Oct 29.
DOI: 10.1002/mabi.202400334
Abstrakt: In this study, the fabrication and characterization of Zn-phthalocyanine/gelatin nanofibrous membranes is reported using the electrospinning technique. The membranes exhibit a homogeneous distribution of Zn-phthalocyanine within the gelatin matrix, maintaining the structural integrity and photosensitizing properties of the phthalocyanine. Scanning electron microscopy revealed that the electrospun fibers possess diameters ranging results as 100-300, 200-700, and 300-800 nm for Gel, ZnPc/Gel 1, and ZnPc/Gel 2, respectively. The addition of ZnPc does not decrease the hydrophilicity of the Gel membrane. The nanofibrous membranes showed good cytocompatibility, as indicated by the high viability of Vero cells exposed to membrane extracts. Furthermore, these composites supported cell adhesion and proliferation on their surfaces. The two Zn-phthalocyanine/gelatin nanofiber formulations exhibited significant antimicrobial activity toward Escherichia Coli (E. Coli) and Staphylococcus Aureus (S. Aureus) under visible light illumination, achieving reductions of 3.4 log 10 and 3.6 log 10 CFU mL -1 for E. coli, and 3.9 log 10 and 4.1 log 10 CFU mL -1 for S. aureus. These results demonstrate the potential of Zn-phthalocyanine/gelatin nanofibrous membranes as effective agents in antibacterial photodynamic therapy, providing a promising solution to control bacterial infections and antibiotic resistance.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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