Acute toxicity of trypsin inhibitor from tamarind seeds in embryo and adult zebrafish ( Danio rerio ).

Autor:	Dos Santos Melo YL; Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Luchiari AC; FishLab, Physiology and Behavior Department, Federal University of Rio Grande do Norte, Natal, RN, Brazil.; Psychobiology Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil., Lopes BS; Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Ferreira Rocha Silva MG; Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil., Dos Santos Pais T; Biochemistry and Molecular Biology Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil., Procópio Gama Cortez JE; Nutrition Course, Federal University of Rio Grande do Norte, Natal, RN, Brazil., da Silva Camillo C; Postgraduate Program in Structural and Functional Biology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil., Bezerra de Moura SA; Postgraduate Program in Structural and Functional Biology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil., da Silva-Maia JK; Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil., de Araújo Morais AH; Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.; Biochemistry and Molecular Biology Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil.
Jazyk:	angličtina
Zdroj:	Toxicology reports [Toxicol Rep] 2024 Oct 10; Vol. 13, pp. 101766. Date of Electronic Publication: 2024 Oct 10 (Print Publication: 2024).
DOI:	10.1016/j.toxrep.2024.101766
Abstrakt:	The trypsin inhibitor isolated from tamarind seeds (TTI) is being investigated for potential applications in the treatment of noncommunicable diseases (NCD), such as hypertension, obesity, and diabetes. This study aimed to assess TTI embryotoxicity and acute toxicity in adult zebrafish ( Danio rerio ). TTI was extracted and isolated from tamarind seeds. Embryonic and adult zebrafish were exposed for 96 hours to three concentrations of TTI (12.5, 25, and 50 mg/L). Zebrafish embryos (n=60 per group) were evaluated for survival, hatching, malformations, and potential developmental marker alterations, in addition to cardiotoxicity and neurotoxicity tests. For acute toxicity assessment in adults (n=20 per group), survival and locomotor and anxiety-like behaviors were assessed, along with genotoxicity (micronucleus) evaluation. Embryos exposed to TTI showed no significant adverse effects, presented normal heart rates and positive reflex response in the neurotoxicity tests. In adult fish, TTI did not cause mortality or significant behavioral changes, suggesting no neurotoxicity and no genotoxicity. Histopathological analyses of the whole body showed only changes in the liver and spinal cord, similar to those observed in the control group not exposed to TTI. These findings indicate TTI's biosafety and therapeutic potential in complex organisms. Further research is required to evaluate its long-term effects and efficacy in treating non-communicable diseases. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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