The function of an apple ATP-dependent Phosphofructokinase gene MdPFK5 in regulating salt stress.

Autor: Zhang LL; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China., Zhu H; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China., Chen CY; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China., Shang NN; Dongying Vocational Institute, Dongying, China., Sheng LX; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China., Yu JQ; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2024 Nov-Dec; Vol. 176 (6), pp. e14590.
DOI: 10.1111/ppl.14590
Abstrakt: Salt stress severely affects the growth and yield of apples (Malus domestica Borkh). Although salt-tolerant genes have been extensively studied, documentation on the role of the ATP-dependent phosphofructokinase gene MdPFK5 in salt stress is limited. This study conducted an evolutionary tree and three-dimensional structure analysis of the PFK gene family in Arabidopsis thaliana and MdPFK (MD01G1037400), revealing a close phylogenetic relationship between MdPFK (MD01G1037400) and AtPFK5. Given the similarity in their protein tertiary structures, MdPFK was designated as MdPFK5, suggesting functional similarities with AtPFK5. Further investigation revealed elevated expression levels of MdPFK5 in apple leaves and flowers, particularly showing significant upregulation 120 days after blooming and differential expression beginning at 3 hours of salt stress. Overexpression of MdPFPK5 conferred salt tolerance in both apple calli and transgenic lines of Arabidopsis thaliana. Moreover, NaCl treatment promoted soluble sugar accumulation in apple calli and transgenic lines of Arabidopsis thaliana overexpressing MdPFK5. This study provides new insights into the salt tolerance function of MdPFK5.
(© 2024 Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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