Effect of green coffee on miR-133a, miR-155 and inflammatory biomarkers in obese individuals.

Autor: Khedr NF; Biochemistry Department, Faculty of Pharmacy, Medical Complex, Tanta University, Al-Baher St, Tanta, 31527, Egypt. naglaa.khedr@pharm.tanta.edu.eg., Zahran ES; Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebeen Elkom, Egypt., Ebeid AM; Clinical Pharmacy Department, Faculty of Pharmacy, AL-Delta University, Gamasa, Egypt., Melek ST; Department of Parasitology and Blood Research at National Organization for Drug Control and Research (NODCAR), 12654, Cairo, Egypt., Werida RH; Clinical Pharmacy Department, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt. rehabwrieda@pharm.dmu.edu.eg.
Jazyk: angličtina
Zdroj: Diabetology & metabolic syndrome [Diabetol Metab Syndr] 2024 Oct 28; Vol. 16 (1), pp. 256. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1186/s13098-024-01478-7
Abstrakt: Objectives: Metabolic syndrome is a cluster of conditions that increases the risk of atherosclerotic cardiovascular diseases. The current study was a randomized, double blind, placebo-controlled study that aimed to determine the impact of green coffee (GC) in obese patients with metabolic syndrome through analysis of miRNA-155, miRNA-133a and the inflammatory biomarkers such as resistin, TNF-α, total sialic acid, homocysteine, high sensitivity C-reactive protein (hs-CRP), and the anti-inflammatory cytokine, adiponectin.
Methods: One hundred-sixty obese patients were randomly supplemented either with GC capsules (800 mg) or placebo daily for six months. Both groups were advised to take a balanced diet. Blood samples were collected at baseline and after six months of supplementation.
Results: GC supplementation for 6 months reduced BMI (p = 0.002), waist circumference (p = 0.038), blood glucose (p = 0.002), HbA1c% (p = 0.000), Insulin (p = 0.000), systolic blood pressure (p = 0.005), diastolic BP (p = 0.001) compared with placebo. GC significantly decreased total cholesterol (TC, p = 0.000), LDL-C (p = 0.001), triglycerides (TG, p = 0.002) and increased HDL-C (p = 0.008) compared with placebo group. In addition, GC significantly (p ≤ 0.005) reduced total sialic acid, homocysteine, resistin, TNF-α, hs-CRP and the oxidative stress marker malondialdehyde (MDA), but increased serum adiponectin (p = 0.000) compared to placebo group. There was a significant reduction in the gene expression of miR-133a (p = 0.000) in GC group as compared with baseline levels and with the control placebo group (p = 0.001) after 6 months.
Conclusion: GC administration modulated metabolic syndrome by decreasing BMI, high BP, blood glucose, dyslipidemia, miRNA-133a and inflammatory biomarkers that constitute risk factors for cardiovascular diseases.
Clinicaltrials: gov registration No. is NCT05688917.
(© 2024. The Author(s).)
Databáze: MEDLINE
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