Loss of myosin light chain kinase induces the cellular senescence associated secretory phenotype to promote breast epithelial cell migration.

Autor: Kim D; Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA. dkim23@fredhutch.org.; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea. dkim23@fredhutch.org., Cooper JA; Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA., Helfman DM; Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 28; Vol. 14 (1), pp. 25786. Date of Electronic Publication: 2024 Oct 28.
DOI: 10.1038/s41598-024-76868-y
Abstrakt: Overexpression or activation of oncogenes or loss of tumor-suppressor genes can induce cellular senescence as a defense mechanism against tumor development, thereby maintaining cellular homeostasis. However, cancer cells can circumvent this senescent state and continue to spread. Myosin light chain kinase (MLCK) is downregulated in many breast cancers. Here we report that downregulation of MLCK in normal breast epithelial cells induces a senescence-associated secretory phenotype and stimulates migration. The reduction of MLCK results in increased p21 Cip1 expression, dependent on p53 and the AKT-mammalian target of rapamycin pathway. Subsequently, p21 Cip1 promotes the secretion of soluble ICAM-1, IL-1α, IL-6 and IL-8, thereby enhancing collective cell migration in a non-cell-autonomous manner. These findings provide new mechanistic insights into the role of MLCK in cellular senescence and cancer progression.
(© 2024. The Author(s).)
Databáze: MEDLINE
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