Characterization of antibiotic resistance development of E. coli in synthetic and real wastewater.

Autor:	Sutradhar I; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA., Gross N; Department of Materials Science and Engineering, Boston University, Boston, MA 02215, USA., Ching C; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA., Nahum Y; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.; Center for Forced Displacement, Boston University, Boston, MA 02215, USA., Desai D; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA., Bowes DA; Center for Forced Displacement, Boston University, Boston, MA 02215, USA.; Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC 29208, USA., Zaman MH; Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.; Center for Forced Displacement, Boston University, Boston, MA 02215, USA.; Center on Emerging Infectious Diseases Research, Boston University, Boston, MA 02118, USA.; Howard Hughes Medical Institute, Boston University, Boston, MA 02215, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 17. Date of Electronic Publication: 2024 Oct 17.
DOI:	10.1101/2024.10.16.618744
Abstrakt:	Antimicrobial resistance (AMR) is a major threat to global health and resistant bacterial populations have been observed to develop and spread in and around wastewater. However, in vitro studies on AMR development are typically conducted in ideal media conditions which can differ in composition and nutrient density from wastewater. In this study, we compare the growth and AMR development of E. coli in standard LB broth to a synthetic wastewater recipe and autoclaved wastewater samples from the Massachusetts Water Resources Authority (MWRA). We found that synthetic wastewater and real wastewater samples both supported less bacterial growth compared to LB. Additionally, bacteria grown in synthetic wastewater and real wastewater samples had differing susceptibility to antibiotic pressure from Doxycycline, Ciprofloxacin, and Streptomycin. However, AMR development over time during continuous passaging under subinhibitory antibiotic pressure was similar in fold change across all media types. Thus, we find that while LB can act as a proxy for wastewater for AMR studies in E. coli , synthetic wastewater is a more accurate predictor of both E.coli growth and antibiotic resistance development. Moreover, we also show that antibiotic resistance can develop in real wastewater samples and components within wastewater likely have synergistic and antagonistic interactions with antibiotics. Competing Interests: Competing Interests Authors have no competing interests to declare.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu