Anticoccidial activities of Piper betle L essential oil on Eimeria tenella oocysts.

Autor: Ristanti R; Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia., Hamid PH; Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia. pennyhumaidahhamid@staff.uns.ac.id.; EcoHealth and Climate Resilience Centre of Tropical Hemisphere, Surakarta, Indonesia. pennyhumaidahhamid@staff.uns.ac.id., Nugroho HA; Research Center for Applied Microbiology, BRIN, Bogor, Indonesia., Nuringtyas TR; Research Center for Biotechnology, Graduate School, Universitas Gadjah Mada, Sleman, Indonesia.; Faculty of Biology, Universitas Gadjah Mada, Sleman, Indonesia., Wibowo S; Eijkman Research Center for Molecular Biology, BRIN, Jakarta, Indonesia., Ferdian PR; Research Center for Applied Zoology, BRIN, Bogor, Indonesia., Rahadian Amalia RL; Research Center for Applied Zoology, BRIN, Bogor, Indonesia., Caro TM; Escuela de Medicina Veterinaria, Facultad de Medicina Veterinaria Y Recursos Naturales, Universidad Santo Tomas, Santiago, Chile.; EcoHealth and Climate Resilience Centre of Tropical Hemisphere, Surakarta, Indonesia., Kusumarini S; Department of Parasitology, Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia., Wardhana AH; EcoHealth and Climate Resilience Centre of Tropical Hemisphere, Surakarta, Indonesia.; Research Center for Veterinary Science, BRIN, Bogor, Indonesia., Matsubayashi M; Faculty of Veterinary Medical Science, Osaka Metropolitan University, Osaka, Japan.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 26; Vol. 14 (1), pp. 25568. Date of Electronic Publication: 2024 Oct 26.
DOI: 10.1038/s41598-024-76754-7
Abstrakt: Coccidiosis poses a significant threat to the poultry industry, with synthetic antibiotics and disinfectants being the primary tools for control. This study investigated the potential of Piper betle L essential oil (PBEO) as a natural alternative against Eimeria tenella, one of the most pathogenic Eimeria species affecting poultry. Our findings revealed that PBEO exhibits significant anticoccidial effects through two primary mechanisms: (i) oocysticidal activity by disintegrating oocyst walls and (ii) inhibition of the sporulation process. PBEO demonstrated oocysticidal activities ranging from 8.67 to 95.33% across concentrations from 0.04 to 40%. Notably, at 72 h post-incubation, a 0.04% PBEO concentration significantly reduced the number of sporulated oocysts (P ≤ 0.05) to 71.67%, showing effects comparable to those of formalin. PBEO reduced 50% of oocyst sporulation (IC 50 ) in the concentration of 1.31% at 72 h. Gas chromatography-mass spectrometry (GC-MS) identified the primary constituents of PBEO, including eugenol, beta-caryophyllene, and other key compounds, collectively constituting 96% of the oil. This research underscores the potential of PBEO as a natural anticoccidial agent and lays the groundwork for further studies aimed at identifying, isolating, and developing active compounds that may specifically target the sporogony process in coccidian parasites.
(© 2024. The Author(s).)
Databáze: MEDLINE
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