A coloaded liposome in situ gel as a novel therapeutic strategy to treat cerebral ischemia reperfusion injury.

Autor: Tian Q; Department of Senile Neurology, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, Shandong, China; Department of Neurology, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China; Medical Integration and Practice Center, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China., Li J; Department of Ophthalmology, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, Shandong, China., Lv R; Medical Image Center (PET/CT), The Affiliated Taian City Centeral Hospital of Qingdao University, Taian 271000, Shandong, China., Zhou G; Department of Senile Neurology, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, Shandong, China., Liu X; Department of Senile Neurology, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, Shandong, China., Wang Y; Department of Senile Neurology, The Affiliated Taian City Central Hospital of Qingdao University, Taian 271000, Shandong, China., Sun B; Department of Neurology, The Second Affiliated Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271000, Shandong, China. Electronic address: blsun88@163.com., Xia Z; Department of Neurology, Liaocheng People's Hospital, Liaocheng 252000, Shandong, China; Department of Neurology, Liaocheng People's Hospital, Shandong University, Jinan 250012, Shandong, China; Department of Neurology, the Second People's Hospital, Liaocheng, Liaocheng 252000, Shandong, China; State Key Laboratory of Dampness Syndrome of Chinese Medicine, Shandong Sub-centre, Liaocheng 252000, Shandong, China. Electronic address: xiazhangyong2013@163.com.
Jazyk: angličtina
Zdroj: Brain research [Brain Res] 2024 Oct 24; Vol. 1847, pp. 149292. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1016/j.brainres.2024.149292
Abstrakt: Background: Ischemic stroke has become one of the leading causes of death and disability worldwide in individuals aged 60 and above. However, currently available drugs show limited efficacy. Therefore, research to find more effective and safer therapeutic strategies is an urgent requirement for the treatment of cerebral ischemia reperfusion injury (CIRI).
Methods: First, the free radical scavenger Edaravone and a Ginseng active ingredient were coloaded into liposomes (aER@Lip), followed by optimization and characterization. Pluronic F127 and F68 at different concentrations were mixed and stored at 4 °C for more than 24 h to obtain gel solutions. Then, aER@Lip was added to the gel solutions to prepare the drug-loaded in situ gel, termed aER@Lip-TSG.
Results: In vitro experiments showed that aER@Lip-TSG was taken up by cells and had a good protective effect on oxygen-glucose deprivation/reoxygenation in pheochromocytoma 12 cells. In a rat CIRI model, aER@Lip-TSG delivered by intranasal administration not only decreased the apoptosis in brain tissue induced by CIRI, but also decreased the resultant inflammatory response. Moreover, the results suggested that aER@Lip-TSG had good biosafety.
Conclusion: This delivery system provides a promising multi-factor combination, synergistic effects, sustained-release capabilities, and is a non-invasive treatment strategy for CIRI. It thus meets the urgent need for effective treatments of central nervous system diseases.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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