Lignocellulose degradation and temperature adaptation mechanisms during composting of mushroom residue and wood chips at low temperature with inoculation of psychrotolerant microbial agent.

Autor: Tian X; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China., Qin X; The College of Life Sciences, Sichuan University, Chengdu, 610041, China., Jia X; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China., Lyu Q; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China., Li S; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China; University of Chinese Academy of Sciences, Beijing, 100049, China., Jiang L; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China; University of Chinese Academy of Sciences, Beijing, 100049, China., Chen L; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China; University of Chinese Academy of Sciences, Beijing, 100049, China., Yan Z; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China., Huang J; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610213, China. Electronic address: huangjun@cib.ac.cn.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Dec 15; Vol. 363 (Pt 2), pp. 125156. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1016/j.envpol.2024.125156
Abstrakt: Low ambient temperature become the limiting factor of composting in cold regions, thus hindering the recycle of agricultural and forestry wastes. In this study, the composting of mushroom residue and wood chips (MRWC) under low temperature was successfully implemented with inoculation of psychrotolerant cellulolytic microbial agent. Composting entered thermophilic stage on third day and the peak temperature reached to 66.25 °C. After 84 days of composting, the degradation rate of cellulose and hemicellulose was 40.85% and 100%, respectively and the compost product was completely mature and met the requirements of organic fertilizer. Metagenomic and transcriptome sequencing were applied to reveal the microbial composition and their substrates conversion functions and adaptation mechanisms through low to high temperatures. Streptomyces, Mesorhizobium, Devosia, Aspergillus and Mucor were dominant genera in the microbial community that were rich in genes of lignocellulose degradation. Various genes related to low temperature adaptation (fatty acid, trehalose, mannitol, betaine metabolism and cold shock mechanism) and high temperature tolerance (heat shock and antioxidant) were detected during MRWC composting. These results indicated that microbes during composting constituted a high-efficiency lignocellulosic ultilization system in cold conditions. Besides, the microbes of microbial agent, especially Streptomyces and Aspergillus, possessed numerous genes involving in lignocellulose degradation and temperature adaptation and quite different temperature response patterns were found to perform in bacteria and fungi. The transcription levels of most these genes in Aspergillus exhibited significant differences under different substrates and temperature conditions, suggesting that the inoculum was crucial to the composting process and beneficial to maintain the temperature of piles. This study demonstrated that the application of psychrotolerant microbes was a promising strategy to increase the efficiency of composting in cold regions and these results could also provided the guidance for optimizing microbial agent.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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