| Autor: |
Vargas-Bermudez DS; Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Bogotá 111321, Colombia., Gil-Silva AC; Zoetis de Colombia, Titan Plaza Centro Empresarial, Bogotá 111021, Colombia., Naranjo-Ortíz MF; Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Bogotá 111321, Colombia., Mogollón JD; Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Bogotá 111321, Colombia., Gómez-Betancur JF; Zoetis de Colombia, Titan Plaza Centro Empresarial, Bogotá 111021, Colombia., Estrada JF; Zoetis de Colombia, Titan Plaza Centro Empresarial, Bogotá 111021, Colombia., Aldaz Á; Zoetis Inc., 10 Sylvan Way, Parsippany, NJ 07054, USA., Garzón-González H; Grupo de Investigación en Ciencias Biomédicas (GICBUPTC), Universidad Pedagógica y Tecnológica de Colombia (UPTC), Tunja 150003, BOY, Colombia., Angulo J; Zoetis Inc., 1040 Swabia Ct, Durham, NC 27703, USA., Foss D; Zoetis Inc., 333 Portage St, Kalamazoo, MI 49007, USA., Gutierrez AH; EpiVax Inc., Providence, RI 02909, USA., Jaime J; Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Bogotá 111321, Colombia. |
| Abstrakt: |
Porcine circovirus type 2 (PCV2) is strongly linked to a group of syndromes referred to as porcine-circovirus-associated diseases (PCVADs), which are controlled through vaccination; however, this does not induce sterilizing immunity but is instead involved in the evolution of the virus and is considered a factor in vaccine failure. This study sampled 84 herds (167 pigs) vaccinated against PCV2 and with clinical signs of PCVADs in five provinces across Colombia. PCV2 was identified and further characterized at the molecular level via genotyping and phylogenetic reconstructions. In addition, PCV2-associated lesions were examined via histopathology. Furthermore, the PCV2-Cap sequences retrieved were compared with three vaccines via the EpiCC tool and T cell epitope coverage. The prevalence of PCV2 was 82% in pigs and 92.9% in herds. The highest viral loads were identified in lymphoid tissue, and PCV2d emerged as the most predominant in pigs and herds (93.4% and 92.3%). Sequences for PCV2-ORF2 ( n = 57; 55 PCV2d and 2 PCV2a) were determined, and PCV2d sequences were highly similar. The most common pneumonia pattern was suppurative bronchopneumonia, while the most common lung lesion was exudation in the airways; in lymphoid tissue, there was lymphoid depletion. The bivalent vaccine (PCV2a and PCVb) exhibited a higher EpiCC score (8.36) and T cell epitope coverage (80.6%) than monovalent PCV2a vaccines. In conclusion, PCV2d currently circulates widely in Colombia. Despite vaccination, there are clinical cases of PCV2, and immunoinformatic analyses demonstrate that bivalent vaccines improved the average coverage. |
