Hepatitis Delta Virus Clade 8 Is the Predominant Clade Circulating in Botswana amongst People Living with HIV.

Autor: Baruti K; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone Private Bag 00704, Botswana., Choga WT; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; School of Allied Health Professions, Faculty of Health Sciences, University of Botswana, Gaborone Private Bag 00704, Botswana., Motshosi PC; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone Private Bag 00704, Botswana., Phinius BB; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; School of Allied Health Professions, Faculty of Health Sciences, University of Botswana, Gaborone Private Bag 00704, Botswana., Phakedi B; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; School of Allied Health Professions, Faculty of Health Sciences, University of Botswana, Gaborone Private Bag 00704, Botswana., Bhebhe LN; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana., Mpebe GGA; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana., Tsayang CD; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana., Ratsoma T; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone Private Bag 00704, Botswana., Gaolathe T; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Faculty of Medicine, University of Botswana, Gaborone Private Bag UB 0022, Botswana., Mosepele M; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Faculty of Medicine, University of Botswana, Gaborone Private Bag UB 0022, Botswana., Makhema J; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA., Shapiro R; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA., Lockman S; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA., Moyo S; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.; Department of Pathology, Division of Medical Virology, Stellenbosch University, Cape Town 7535, South Africa.; School of Health Systems and Public Health, University of Pretoria, Pretoria 0002, South Africa., Jongman M; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Biological Sciences, Faculty of Science, University of Botswana, Gaborone Private Bag 00704, Botswana., Anderson M; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Africa Health Research Institute (AHRI), Durban 4013, South Africa.; The Francis Crick Institute, London NW1 2BE, UK., Gaseitsiwe S; Research Laboratory, Botswana Harvard Health Partnership, Gaborone Private Bag BO 320, Botswana.; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 Oct 04; Vol. 16 (10). Date of Electronic Publication: 2024 Oct 04.
DOI: 10.3390/v16101568
Abstrakt: Hepatitis delta virus (HDV) co-infections more often result in severe hepatitis compared to hepatitis B virus (HBV) infections alone. Despite a high HDV prevalence (7.1%), information regarding circulating HDV clades is very limited in Botswana. We extracted total nucleic acid from confirmed HDV-positive samples and quantified their viral load. We then sequenced the large hepatitis delta antigen (L-HDAg) using Oxford Nanopore Technology (ONT). Genotyping was performed using the HDV Database, and HDV mutation profiling was performed on AliView. All participants with HBV genotypic information belonged to sub-genotype A1, and 80% (4/5) of them had a higher HDV viral load and a lower HBV viral load. We sequenced 75% (9/12) of the HDV-positive samples, which belonged to HDV clade 8. A total of 54 mutations were discovered, with the most prevalent being Q148R (16%), D149P (16%) and G151D (16%). Known mutations such as S117A, K131R, R139K and G151D were detected, while the other mutations were novel. Our results reveal that HDV clade 8 is the predominant clade in Botswana. The significance of all mutations remains unclear. Future studies with a larger sample size to detect other HDV clades that might be circulating in Botswana and functionally characterize the detected mutations are warranted.
Databáze: MEDLINE
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