| Autor: |
Dican L; Department of Medical Biochemistry, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania.; Clinical Institute of Urology and Renal Transplantation, 400000 Cluj-Napoca, Romania., Iancu M; Department of Medical Informatics and Biostatistics, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Elec FI; Clinical Institute of Urology and Renal Transplantation, 400000 Cluj-Napoca, Romania.; Department of Urology, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Burghelea D; Clinical Institute of Urology and Renal Transplantation, 400000 Cluj-Napoca, Romania., Timoce R; Clinical Institute of Urology and Renal Transplantation, 400000 Cluj-Napoca, Romania., Catana CS; Department of Medical Biochemistry, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Marta MM; Department of Medical Education, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Lucaciu RL; Department of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Hangan AC; Department of Inorganic Chemistry, Faculty of Pharmacy, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Matei HV; Department of Cellular and Molecular Biology, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania., Loga LI; Clinical Institute of Urology and Renal Transplantation, 400000 Cluj-Napoca, Romania.; Department of Cellular and Molecular Biology, Faculty of Medicine, 'Iuliu-Hațieganu' University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. |
| Abstrakt: |
The HLA profile is essential in cell and tissue transplantation, particularly in patients with autoimmune conditions and infections. Due to the extreme polymorphism in certain HLA loci, it also serves as a key tool for population genetic analysis. This study aimed to identify the allele and haplotype distributions of HLA class I (A, B, and C) and class II (DRB1) genotypes in unrelated hematopoietic stem cell donors. A retrospective analysis was conducted between 2016 and 2020 on 9832 Transylvanian volunteers, divided into Romanian and Hungarian groups based on self-reported ethnicity. Using PCR-SSO for HLA typing, significant differences were found in allele frequencies between ethnic groups. A total of 19 HLA-A, 31 HLA-B, 14 HLA-C, and 13 HLA-DRB1 distinct allele groups were identified between ethnic groups. Notably, B*18, B*51, and C*12 were more frequent in Romanians, while B*44, B*40, and C*07 were more common in Hungarians. Differences in haplotype distributions were also observed, with HLA-A*02~B*18~C*07~DRB1*11 being significantly more frequent in Romanians. Understanding these population-specific HLA profiles can improve donor matching for hematologic diseases, enhancing patient outcomes and access to life-saving hematopoietic stem cell transplantation. |
