A Novel Hydrogel Sponge for Three-Dimensional Cell Culture.

Autor: Baldassari S; Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy., Yan M; The Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518000, China., Ailuno G; Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy., Zuccari G; Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy., Bassi AM; Department of Experimental Medicine (DIMES), University of Genoa, 16132 Genoa, Italy.; Inter-University Center for the Promotion of the 3Rs Principles in Teaching & Research (Centro 3R), 56122 Pisa, Italy., Vernazza S; Department of Experimental Medicine (DIMES), University of Genoa, 16132 Genoa, Italy.; Inter-University Center for the Promotion of the 3Rs Principles in Teaching & Research (Centro 3R), 56122 Pisa, Italy., Tirendi S; Department of Experimental Medicine (DIMES), University of Genoa, 16132 Genoa, Italy.; Inter-University Center for the Promotion of the 3Rs Principles in Teaching & Research (Centro 3R), 56122 Pisa, Italy., Ferrando S; Laboratory of Comparative Anatomy, Department of Earth, Environmental, and Life Sciences (DISTAV), University of Genoa, 16132 Genoa, Italy., Comite A; Department of Chemistry and Industrial Chemistry (DCCI), University of Genoa, 16146 Genoa, Italy., Drava G; Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy., Caviglioli G; Department of Pharmacy (DIFAR), University of Genoa, 16148 Genoa, Italy.; IRCCS Ospedale Policlinico San Martino, 16132 Genoa, Italy.
Jazyk: angličtina
Zdroj: Pharmaceutics [Pharmaceutics] 2024 Oct 19; Vol. 16 (10). Date of Electronic Publication: 2024 Oct 19.
DOI: 10.3390/pharmaceutics16101341
Abstrakt: Background/objectives: Three-dimensional (3D) cell culture technologies allow us to overcome the constraints of two-dimensional methods in different fields like biochemistry and cell biology and in pharmaceutical in vitro tests. In this study, a novel 3D hydrogel sponge scaffold, composed of a crosslinked polyacrylic acid forming a porous matrix, has been developed and characterized.
Methods: The scaffold was obtained via an innovative procedure involving thermal treatment followed by a salt-leaching step on a matrix-containing polymer along with a gas-forming agent. Based on experimental design for mixtures, a series of formulations were prepared to study the effect of the three components (polyacrylic acid, NaHCO 3 and NaCl) on the scaffold mechanical properties, density, swelling behavior and morphological changes. Physical appearance, surface morphology, porosity, molecular diffusion, transparency, biocompatibility and cytocompatibility were also evaluated.
Results: The hydrogel scaffolds obtained show high porosity and good optical transparency and mechanical resistance. The scaffolds were successfully employed to culture several cell lines for more than 20 days.
Conclusions: The developed scaffolds could be an important tool, as such or with a specific coating, to obtain a more predictive cellular response to evaluate drugs in preclinical studies or for testing chemical compounds, biocides and cosmetics, thus reducing animal testing.
Databáze: MEDLINE
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