Prognostic Impact of H19/Cell Adhesion Molecules Circuitry on Prostate Cancer Biopsy.

Autor:	Pecci V; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Pierconti F; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy.; Department of Woman, Child and Public Health, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Carlino A; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy., Pinto F; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy., Gradilone U; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., De Martino S; National Research Council (CNR)-Istituto di Analisi dei Sistemi ed Informatica 'Antonio Ruberti' (IASI), 00185 Rome, Italy., Rotili D; Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, 00185 Rome, Italy., Grassi C; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy.; Department of Neuroscience, Università Cattolica del Sacro Cuore, 00168 Rome, Italy., Pontecorvi A; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy., Gaetano C; Laboratory of Epigenetics, Istituti Clinici Scientifici Maugeri IRCCS, 27100 Pavia, Italy., Strigari L; Department of Medical Physics, S. Orsola Malpighi University Hospital, 40138 Bologna, Italy., Farsetti A; National Research Council (CNR)-Istituto di Analisi dei Sistemi ed Informatica 'Antonio Ruberti' (IASI), 00185 Rome, Italy., Nanni S; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.; Fondazione 'Policlinico Universitario A. Gemelli IRCCS', 00168 Rome, Italy.
Jazyk:	angličtina
Zdroj:	Biomedicines [Biomedicines] 2024 Oct 12; Vol. 12 (10). Date of Electronic Publication: 2024 Oct 12.
DOI:	10.3390/biomedicines12102322
Abstrakt:	Introduction: Metastatic prostate cancer (PCa) presents a significant challenge in oncology due to its high mortality rate and the absence of effective biomarkers for predicting patient outcomes. Building on previous research that highlighted the critical role of the long noncoding RNA (lncRNA) H19 and cell adhesion molecules in promoting tumor progression under hypoxia and estrogen stimulation, this study aimed to assess the potential of these components as prognostic biomarkers for PCa at the biopsy stage. Methods: This research utilized immunohistochemistry and droplet digital PCR to analyze formalin-fixed paraffin-embedded (FFPE) biopsies, focusing on specific markers within the H19/cell adhesion molecules pathway. Results: A novel multivariate analysis led to a "BioScore", a composite biomarker score to predict disease progression. This score is based on evaluating five key markers: the expression levels of Hypoxia-Inducible Factor 2 Alpha (HIF-2α), endothelial Nitric Oxide Synthase (eNOS), β4 integrin, E-cadherin transcript (CDH1), and lncRNA H19. The criteria for the "BioScore" involve identifying three out of these five markers, combining elevated levels of HIF-2α, eNOS, β4 integrin, and CDH1 with reduced H19 expression. Conclusions: This finding suggests the possibility of identifying, at the time of biopsy, PCa patients at higher risk of metastasis based on dysregulation in the H19/cell adhesion molecules circuitry. This study provides a valuable opportunity for early intervention in managing PCa, potentially contributing to personalized treatment strategies.
Databáze:	MEDLINE
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