| Autor: |
Bozó R; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary.; HCEMM-USZ Skin Research Group, University of Szeged, H-6720 Szeged, Hungary., Flink LB; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary.; HCEMM-USZ Skin Research Group, University of Szeged, H-6720 Szeged, Hungary., Ambrus B; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary., Ghaffarinia A; HCEMM-USZ Skin Research Group, University of Szeged, H-6720 Szeged, Hungary., Koncz B; Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, H-6726 Szeged, Hungary.; HCEMM-BRC Systems Immunology Research Group, H-6726 Szeged, Hungary., Kui R; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary., Gyulai R; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary., Kemény L; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary.; HCEMM-USZ Skin Research Group, University of Szeged, H-6720 Szeged, Hungary.; HUN-REN-SZTE Dermatological Research Group, Hungarian Research Network, H-6720 Szeged, Hungary., Bata-Csörgő Z; Department of Dermatology and Allergology, Albert Szent-Györgyi Medical School, University of Szeged, H-6720 Szeged, Hungary.; HCEMM-USZ Skin Research Group, University of Szeged, H-6720 Szeged, Hungary.; HUN-REN-SZTE Dermatological Research Group, Hungarian Research Network, H-6720 Szeged, Hungary. |
| Abstrakt: |
In the psoriatic non-lesional (PS-NL) skin, the tissue environment potentially influences the development and recurrence of lesions. Therefore, we aimed to investigate mechanisms involved in regulating tissue organization in PS-NL skin. Cytokine, chemokine, protease, and protease inhibitor levels were compared between PS-NL skin of patients with mild and severe symptoms and healthy skin. By comparing mild and severe PS-NL vs. healthy skin, differentially expressed cytokines and chemokines suggested alterations in hemostasis-related processes, while protease inhibitors showed no psoriasis severity-related changes. Comparing severe and mild PS-NL skin revealed disease severity-related changes in the expression of proteases, cytokines, and chemokines primarily involving methyl-CpG binding protein 2 (MECP2) and extracellular matrix organization-related mechanisms. Cytokine and chemokine expression in clinically resolved versus healthy skin showed slight interleukin activity, differing from patterns in mild and severe PS-NL skin. Immunofluorescence analysis revealed the severity-dependent nuclear expression pattern of MECP2 and decreased expression of 5-methylcytosine and 5-hydroxymethylcytosine in the PS-NL vs. healthy skin, and in resolved vs. healthy skin. Our results suggest distinct cytokine-chemokine signaling between the resolved and PS-NL skin of untreated patients with varying severities. These results highlight an altered inflammatory response, epigenetic regulation, and tissue organization in different types of PS-NL skin with possibly distinct, severity-dependent para-inflammatory states. |
