| Autor: |
Mladenić T; Department of Medical Biology and Genetics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Wagner J; Department of Medical Biology and Genetics, Faculty of Medicine, Josip Juraj Strossmayer University, 31000 Osijek, Croatia., Kadivnik M; Department of Obstetrics and Gynecology, Faculty of Medicine, Josip Juraj Strossmayer University, 31000 Osijek, Croatia.; Clinic of Obstetrics and Gynecology, University Hospital Center Osijek, 31000 Osijek, Croatia., Pereza N; Department of Medical Biology and Genetics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Ostojić S; Department of Medical Biology and Genetics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia., Peterlin B; Clinical Institute of Medical Genetics, University Medical Center Ljubljana, 1000 Ljubljana, Slovenia., Dević Pavlić S; Department of Medical Biology and Genetics, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia. |
| Abstrakt: |
This study investigated the potential role of specific single-nucleotide polymorphisms (SNPs) in the genes Astrotactin 1 ( ASTN1 ), EBF Transcription Factor 1 ( EBF1 ), Eukaryotic Elongation Factor , Selenocysteine-tRNA Specific ( EEFSEC ), Microtubule-Associated Serine/Threonine Kinase 1 ( MAST1 ), and Tumor Necrosis Factor Alpha ( TNF-α ) to assess whether these genetic variants contribute to the risk of spontaneous preterm birth (sPTB). A case-control study was conducted involving 573 women from Croatia and Slovenia: 248 with sporadic sPTB (positive personal and negative family history of sPTB before 37 weeks' gestation), 44 with familial sPTB (positive personal and family history of sPTB before 37 weeks' gestation), and 281 control women. The analysis of ASTN1 rs146756455, EBF1 rs2963463, EBF1 rs2946169, EEFSEC rs201450565, MAST1 rs188343966, and TNF-α rs1800629 SNPs was performed using TaqMan real-time PCR. p -values were Bonferroni-adjusted for multiple comparisons. EBF1 SNP rs2963463 was significantly associated with sPTB ( p adj = 0.03). Women carrying the CC genotype had a 3-4-times lower risk of sPTB ( p adj < 0.0001). In addition, a significant difference in the frequency of the minor C allele was observed when comparing familial sPTB cases with controls ( p adj < 0.0001). All other associations were based on unadjusted p -values. The minor T allele of EBF1 SNP rs2946169 was more frequent in sPTB cases overall than in controls, especially in sporadic sPTB ( p = 0.045). Similarly, the CC genotype of ASTN1 SNP rs146756455 was more frequent in sporadic sPTB cases compared to controls ( p = 0.019). Finally, the TNF-α SNP rs1800629 minor A allele and AA genotype were more common in the familial sPTB group compared to sporadic sPTB and controls ( p < 0.05). The EBF1 SNP rs2963463 polymorphism showed a protective effect in the pathogenesis of sPTB, particularly in women carrying the CC genotype. Moreover, EBF1 SNP rs2946169 and ASTN1 SNP rs146756455, as well as TNF-α SNP rs1800629, were associated with an increased risk of sPTB, representing suggestive potential risk factors for sporadic and familial sPTB, respectively. |
