| Autor: |
Mamadalieva NZ; Institute of the Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Mirzo Ulugbek Str. 77, Tashkent 100170, Uzbekistan.; Tashkent Institute of Irrigation and Agricultural Mechanization Engineers, National Research University, Kori Niyazov Str. 39, Tashkent 100000, Uzbekistan.; Department of Pharmacy and Chemistry, Faculty of Medicine, Alfraganus University, Tashkent 100190, Uzbekistan.; Translational Research Center in Oncohaematology, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland., Koval A; Translational Research Center in Oncohaematology, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland., Dusmuratov MM; Department of Organic Synthesis, Faculty of Industrial Pharmacy, Tashkent Pharmaceutical Institute, Oybek Str. 45, Tashkent 100015, Uzbekistan., Hussain H; International Joint Laboratory of Medicinal Food Development and Health Products Creation, Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze 274000, China., Katanaev VL; Translational Research Center in Oncohaematology, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland. |
| Abstrakt: |
A comprehensive metabolite profiling of the medicinal plant Silene viridiflora using an UHPLC-ESI-MS/MS method is described for the first time. A total of 71 compounds were identified and annotated, the most common of which were flavonoids, triterpene glycosides, and ecdysteroids. The three major compounds schaftoside, 26-hydroxyecdysone, and silviridoside can be chosen as the markers for the assessment of the quality of S. viridiflora preparations. The methanol extract and a variety of metabolites identified in S. viridiflora were screened for their cytotoxic and Wnt pathway-inhibiting activities against triple-negative breast cancer (TNBC), the deadliest form of cancer in women. 2-Deoxy-20-hydroxyecdysone with submicromolar IC 50 was identified as a result. The structure-activity relationship derived from the data from the in vitro proliferation assay showed that the hydroxyl group present at position C-2 of steroid core reduces the ecdysteroids' cytotoxicity against cancer cells. |
