| Autor: |
Hwang S; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea.; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea., Park MS; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea.; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea., Koo AJ; College of Engineering, North Carolina A&T State University, Greensboro, NC 27411, USA., Yoo E; Chemical, Biological, and Bioengineering Department, North Carolina A&T State University, Greensboro, NC 27411, USA., Song SH; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea., Kim HK; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea.; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea., Park MH; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea.; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea., Kang JS; College of Pharmacy, Kyungsung University, 309 Suyeong-ro, Busan 48434, Republic of Korea.; Brain Busan 21 Plus Research Project Group, Kyungsung University, Busan 48434, Republic of Korea. |
| Abstrakt: |
Platelets are essential blood components that maintain hemostasis, prevent excessive bleeding, and facilitate wound healing. Reduced platelet counts are implicated in various diseases, including leukemia, hepatitis, cancer, and Alzheimer's disease. Enhancing megakaryocytic differentiation is a promising strategy to increase platelet production. Compound K (CK), a major bioactive metabolite of ginsenosides from Panax ginseng , has demonstrated anti-cancer and neuroprotective properties. In this study, we investigated the effects of CK on megakaryocytic differentiation and apoptosis in chronic myeloid leukemia (CML) cell lines K562 and Meg-01. CK treatment significantly upregulated the mRNA expression of key megakaryocytic differentiation markers, including CD61, CD41, and CD42a, and promoted the formation of large, multinucleated cells in K562 cells. Additionally, flow cytometry analysis revealed that CK at 5 µM induced apoptosis, a critical process in thrombocytopoiesis, in both K562 and Meg-01 cells. RT 2 Profiler PCR array analysis further identified a marked increase in the expression of genes associated with the activation of the NLRP3 inflammasome in CK-treated K562 and Meg-01 cells. This study is the first to demonstrate that CK promotes megakaryocytic differentiation and apoptosis through the activation of the ERK/EGR1 and NLRP3 inflammasome pathways. These findings suggest that CK may enhance platelet production, indicating its potential as a therapeutic candidate for platelet-related disorders and other associated diseases. |
