Polygoni Cuspidati Rhizoma et Radix extract activates TFEB and alleviates hepatic steatosis by promoting autophagy.

Autor: Li C; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China., Li C; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China., Wang Y; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China., You S; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai 200030, China., Man KY; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China., Fan Z; Institute of High Energy Physics, CAS, Beijing 100000, China; Spallation Neutron Source Science Center, CAS, Dongguan, Guangdong 523000, China., Yu Q; Tumor Immunology and Cytotherapy of Medical Research Center, Center for GI Cancer Diagnosis and Treatment, the Affiliated Hospital of Qingdao University, Qingdao 266000, China., Zhang M; Shenzhen Research Institute, The Hong Kong Polytechnic University, Hong Kong, China., Cheng KK; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hong Kong, China; Shenzhen Research Institute, The Hong Kong Polytechnic University, Hong Kong, China; Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University, Hong Kong, China., Mok DK; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China; Shenzhen Research Institute, The Hong Kong Polytechnic University, Hong Kong, China; Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University, Hong Kong, China., Chan SW; Department of Food and Health Sciences, Technological and Higher Education Institute of Hong Kong, Hong Kong, China., Zhang H; Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong, China; Shenzhen Research Institute, The Hong Kong Polytechnic University, Hong Kong, China; Research Centre for Chinese Medicine Innovation (RCMI), The Hong Kong Polytechnic University, Hong Kong, China. Electronic address: zhanghuan.zhang@polyu.edu.hk.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2024 Dec 15; Vol. 359, pp. 123158. Date of Electronic Publication: 2024 Oct 23.
DOI: 10.1016/j.lfs.2024.123158
Abstrakt: Hepatic steatosis, characterized by excessive lipid accumulation in the liver, can be ameliorated by autophagy activation. Polygoni Cuspidati rhizome et Radix (PCRR), traditionally used to treat atherosclerosis, hepatitis, and gallstones, has recently demonstrated anti-steatotic effects in the liver. However, the active compounds and underlying mechanisms remain unclear. This study investigated whether PCRR water extract improves steatosis by modulating hepatic autophagic flux. We found that PCRR water extract promoted autophagic flux, enhanced lysosomal biogenesis, and alleviated lipid accumulation in the liver cell lines as well as in the livers of rats with steatosis. Mechanistically, PCRR water extract inhibited mechanistic target of rapamycin complex 1 (mTORC1) activity, leading to dephosphorylation and subsequent nuclear translocation of transcription factor EB (TFEB), a key regulator of lipophagy. TFEB knockdown attenuated PCRR-mediated lipophagy promotion in the liver cell lines. Furthermore, chloroquine (CQ)-mediated autophagy blockage abrogated the therapeutic effect of PCRR against hepatic steatosis in high-fat diet (HFD)-fed rats. These findings suggest that PCRR water extract acts as a novel autophagy enhancer and holds therapeutic potential for hepatic steatosis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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