Pongamia pinnata L. seed-derived karanjin as prominent antiviral agent against Newcastle disease virus.

Autor: Singh R; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address: S.RASHMI@IITG.AC.IN., Kumar S; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address: SACHINKU@IITG.AC.IN., Rangan L; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address: LRANGAN@IITG.AC.IN.
Jazyk: angličtina
Zdroj: Virology [Virology] 2024 Dec; Vol. 600, pp. 110272. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1016/j.virol.2024.110272
Abstrakt: Current study aim to explore Karanjin (Kar) isolated from the seeds of Pongamia pinnata as a potential antiviral polyphenol against Newcastle disease virus (NDV) through comprehensive investigations. For the in vitro study, plaque assays, gene and protein expression were used to analyse the inhibitory impact of Kar on NDV, where it reduced NDV replication as shown by a 13-fold suppression of the hemagglutinin-neuraminidase (HN) gene and decrease about 60% in virus activity. In ovo study showed that Kar mitigates NDV effects in chicken embryos. In silico studies involving molecular docking and molecular dynamics simulations showed strong binding between Kar and HN protein of NDV. Kar also influenced glucose metabolism, enhancing antiviral responses, as showed by the upregulation of GLUT1 and HEX genes through RT-qPCR and HPLC analyses. The study contributes valuable insights for future investigations into therapeutic applications of Kar against viral infections.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: