Evaluation effect of alginate hydrogel containing losartan on wound healing and gene expression.

Autor: Zamani S; Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Salehi M; Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.; Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.; Sexual Health and Fertility Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.; Health Technology Incubator Center, Shahroud University of Medical Sciences, Shahroud, Iran., Abbaszadeh-Goudarzi G; Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Cheraghali D; Department of Mechanical Engineering, New Jersey Institute of Technology, Newark, NJ, USA., Ehterami A; Institute for Regenerative Medicine (IREM), University of Zurich, Zurich, Switzerland., Esmaili S; Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran., Rezaei Kolarijani N; Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Jazyk: angličtina
Zdroj: Journal of biomaterials applications [J Biomater Appl] 2024 Oct 25, pp. 8853282241292144. Date of Electronic Publication: 2024 Oct 25.
DOI: 10.1177/08853282241292144
Abstrakt: Skin tissue engineering has become an increasingly popular alternative to conventional treatments for skin injuries. Hydrogels, owing to their advantages have become the ideal option for wound dressing, and they are extensively employed in a mixture of different drugs to accelerate wound healing. Sodium alginate is a readily available natural polymer with advantages such as bio-compatibility and a non-toxicological nature that is commonly used in hydrogel form for medical applications such as wound repair and drug delivery in skin regenerative medicine. Losartan is a medicine called angiotensin receptor blocker (ARB) that can prevent fibrosis by inhibiting AT 1 R (angiotensin II type 1 receptor). In this research, for the first time, three-dimensional scaffolds based on cross-linked alginate hydrogel with CaCl 2 containing different concentrations of losartan for slow drug release and exudate absorption were prepared and characterized as wound dressing. Alginate hydrogel was mixed with 10, 1, 0.1, and 0.01 mg/mL of losartan, and their properties such as morphology, chemical structure, water uptake properties, biodegradability, stability assay, rheology, blood compatibility, and cellular response were evaluated. In addition, the therapeutic efficiency of the developed hydrogels was then assessed in an in vitro wound healing model and with a gene expression. The results revealed that the hydrogel produced was very porous (porosity of 47.37 ± 3.76 µm) with interconnected pores and biodegradable (weight loss percentage of 60.93 ± 4.51% over 14 days). All hydrogel formulations have stability under various conditions. The use of CaCl 2 as a cross-linker led to an increase in the viscosity of alginate hydrogels. An in vitro cell growth study revealed that no cytotoxicity was observed at the suggested dosage of the hydrogel. Increases in Losartan dosage, however, caused hemolysis. In vivo study in adult male rats with a full-thickness model showed greater than 80% improvement of the primary wound region after 2 weeks of treatment with alginate hydrogel containing 0.1 mg/mL Losartan. RT-PCR and immunohistochemistry analysis showed a decrease in expression level of TGF-β 1 and VEGF in treatment groups. Histological analysis demonstrated that the alginate hydrogel containing Losartan can be effective in wound repair by decreasing the size of the scar and tissue remodeling, as evidenced by future in vivo studies.
Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze: MEDLINE
Externí odkaz: