Border-like cell formation mediated by SgPG1 confers aluminum resistance in Stylosanthes guianensis.

Autor: Lin Y; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Liu G; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Liu P; Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, P. R. China., Chen Q; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Guo X; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Lu X; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Cai Z; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Sun L; Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China., Liu J; Robert Holley Center, US Department of Agriculture, Agricultural Research Service, Cornell University, Ithaca, New York, 14853, USA., Chen K; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Liu G; Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agriculture Sciences, Haikou, 571101, P. R. China., Tian J; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China., Liang C; Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, 510642, P. R. China.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Nov; Vol. 120 (4), pp. 1605-1624. Date of Electronic Publication: 2024 Oct 25.
DOI: 10.1111/tpj.17073
Abstrakt: Stylosanthes is an important forage legume in tropical areas with strong resistance to aluminum (Al) toxicity, though knowledge of mechanisms underlying this resistance remains fragmentary. We found that border-like cells (BLCs) were constitutively produced surrounding the root tips of all 54 examined Stylosanthes guianensis genotypes, but not the Stylosanthes viscose genotype TF0140. In genotypic comparisons under Al conditions, the S. guianensis genotype RY#2 retained significantly more Al in BLCs and thereby showed higher relative root growth than TF0140. Formation of BLCs accompanied changes in cell wall pectin epitopes and differential expression of genes involved in pectin metabolism, including a polygalacturonase (SgPG1). The expression pattern of SgPG1 was consistent with the formation of BLCs in both RY#2 and TF0140. SgPG1 was localized in cell walls and exhibited high activities mediating demethyl-esterified homogalacturonan degradation. Overexpressing SgPG1 changed cell wall pectin epitopes, enhanced BLCs production, and Al resistance in both Arabidopsis and Stylosanthes hairy roots. Furthermore, combining protein-DNA binding assays in vitro and in vivo, a bHLH transcription factor SgbHLH19 was demonstrated to be the upstream regulator of SgPG1. Our study demonstrates that S. guianensis Al resistance mainly relies on BLCs, whose formation involves cell wall pectin epitope modification by SgPG1.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: