Antiproliferative Effects of Naja anchietae and Naja senegalensis Venom Peptides on Glioblastoma Cell Lines.

Autor: Boughanmi Y; Aix Marseille University, Institut de Chimie Radicalaire UMR 7273, 13397 Marseille, France.; Latoxan, 26800 Portes-lès-Valence, France., Berenguer-Daizé C; Aix-Marseille University, INP-Institute of Neuropathophysiology-UMR 7051 CNRS, 13005 Marseille, France., Balzano M; BioCytex 140 Chemin de l'Armée d'Afrique, 13010 Marseille, France., Mosrati H; Aix Marseille University, Institut de Chimie Radicalaire UMR 7273, 13397 Marseille, France., Moulard M; BioCytex 140 Chemin de l'Armée d'Afrique, 13010 Marseille, France., Mansuelle P; Proteomics Platform, Marseille Proteomics (MaP), Institut de Microbiologie de la Méditerranée (IMM), FR 3479, CNRS, 13009 Marseille, France., Fourquet P; Aix-Marseille University, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Proteomics, 13009 Marseille, France., Torre F; Aix-Marseille University, Institut Méditerranéen de Biodiversité et d'Ecologie (IMBE), Avignon University, CNRS, IRD, 13397 Marseille, France., de Pomyers H; Latoxan, 26800 Portes-lès-Valence, France., Gigmes D; Aix Marseille University, Institut de Chimie Radicalaire UMR 7273, 13397 Marseille, France., Ouafik L; Aix-Marseille University, INP-Institute of Neuropathophysiology-UMR 7051 CNRS, 13005 Marseille, France., Mabrouk K; Aix Marseille University, Institut de Chimie Radicalaire UMR 7273, 13397 Marseille, France.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2024 Oct 10; Vol. 16 (10). Date of Electronic Publication: 2024 Oct 10.
DOI: 10.3390/toxins16100433
Abstrakt: This study explores the potential of natural bioactive peptides from animal venoms as targeted anti-cancer agents with reduced toxicity. Initially, we screened a broad collection of animal venoms for their antiproliferative activity against cancer cell lines. From this collection, we selected venoms from Naja anchietae and Naja senegalensis due to their promising activity. Utilizing reverse- phase high-performance liquid chromatography (RP HPLC), mass spectrometry (MALDI-TOF MS and MALDI-TOF TOF MSMS), and Edman degradation sequencing, we isolated and characterized three peptides named CTNanc1, CTNanc2, and CTNanc3 from Naja anchietae , and three others named CTNsen1, CTNsen2, and CTNsen3 from Naja senegalensis , each with a molecular weight of around 7 kDa. These purified peptides demonstrated inhibition of U87 glioblastoma cell proliferation, but not of U251 and T98G cells, in cell viability assays. To assess the impact of these treatments on cell viability, apoptosis, and necrosis, flow cytometry assays were conducted on U87 cells at 72 h. The results showed a decrease in cell viability and an increase in dead cells, suggesting that the treatments not only promote apoptosis, but may also lead to increased necrosis or late-stage apoptosis as the exposure time increases. These findings suggest that these peptides could be developed as leads for cancer therapy.
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje