Comparing Different Methods for the Diagnosis of Liver Steatosis: What Are the Best Diagnostic Tools?

Autor: Chopinet S; Department of Digestive Surgery and Liver Transplantation, Hôpital la Timone, AP-HM, 13005 Marseille, France.; Aix Marseille Université, LIIE, 13007 Marseille, France.; Aix Marseille Université, CERIMED, 13007 Marseille, France., Lopez O; Aix Marseille Université, LIIE, 13007 Marseille, France.; Aix Marseille Université, CERIMED, 13007 Marseille, France.; Department of Digestive and Oncologic Radiology, Hôpital l'Archet 2, 06202 Nice, France., Brustlein S; Aix Marseille Université, CERIMED, 13007 Marseille, France., Uzel A; INSA-Lyon, Université Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR5220, U1294, 69616 Lyon, France., Moyon A; C2VN, INSERM 1263 INRAE 1260, Aix-Marseille Université, 13007 Marseille, France.; Department of Radiopharmacy, Hôpital la Timone, AP-HM, 13007 Marseille, France., Varlet I; Aix-Marseille Université, CNRS, CRMBM, 13007 Marseille, France., Balasse L; Aix Marseille Université, CERIMED, 13007 Marseille, France., Kober F; Aix-Marseille Université, CNRS, CRMBM, 13007 Marseille, France., Bobot M; Aix Marseille Université, CERIMED, 13007 Marseille, France.; C2VN, INSERM 1263 INRAE 1260, Aix-Marseille Université, 13007 Marseille, France.; Center of Nephrology and Kidney Transplantation, Hôpital de la Conception, AP-HM, 13005 Marseille, France., Bernard M; Aix-Marseille Université, CNRS, CRMBM, 13007 Marseille, France., Haffner A; Department of Anatomopathology, Hôpital la Timone, AP-HM, 13007 Marseille, France., Sdika M; INSA-Lyon, Université Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR5220, U1294, 69616 Lyon, France., Montcel B; INSA-Lyon, Université Lyon 1, UJM-Saint Etienne, CNRS, Inserm, CREATIS UMR5220, U1294, 69616 Lyon, France., Guillet B; Aix Marseille Université, CERIMED, 13007 Marseille, France.; C2VN, INSERM 1263 INRAE 1260, Aix-Marseille Université, 13007 Marseille, France.; Department of Radiopharmacy, Hôpital la Timone, AP-HM, 13007 Marseille, France., Vidal V; Aix Marseille Université, LIIE, 13007 Marseille, France.; Aix Marseille Université, CERIMED, 13007 Marseille, France.; Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille, France., Grégoire E; Aix Marseille Université, LIIE, 13007 Marseille, France.; Aix Marseille Université, CERIMED, 13007 Marseille, France., Hardwigsen J; Department of Digestive Surgery and Liver Transplantation, Hôpital la Timone, AP-HM, 13005 Marseille, France.; Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille, France., Brige P; Aix Marseille Université, LIIE, 13007 Marseille, France.; Aix Marseille Université, CERIMED, 13007 Marseille, France.
Jazyk: angličtina
Zdroj: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2024 Oct 16; Vol. 14 (20). Date of Electronic Publication: 2024 Oct 16.
DOI: 10.3390/diagnostics14202292
Abstrakt: Background: Due to the ongoing organ shortage, marginal grafts with steatosis are more frequently used in liver transplantation, leading to higher occurrences of graft dysfunction. A histological analysis is the gold standard for the quantification of liver steatosis (LS), but has its drawbacks: it is an invasive method that varies from one pathologist to another and is not available in every hospital at the time of organ procurement. This study aimed to compare non-invasive diagnostic tools to a histological analysis for the quantification of liver steatosis.
Methods: Male C57BL6J mice were fed with a methioninecholine-deficient (MCD) diet for 14 days or 28 days to induce LS, and were compared to a control group of animals fed with a normal diet. The following non-invasive techniques were performed and compared to the histological quantification of liver steatosis: magnetic resonance spectroscopy (MRS), CARS microscopy, 99m Tc MIBI SPECT imaging, and a new near-infrared spectrometer (NIR-SG1).
Results: After 28 days on the MCD diet, an evaluation of LS showed ≥30% macrovesicular steatosis. High correlations were found between the NIR-SG1 and the blinded pathologist analysis (R 2 = 0.945) ( p = 0.001), and between the CARS microscopy (R 2 = 0.801 ( p < 0.001); MRS, R 2 = 0.898 ( p < 0.001)) and the blinded pathologist analysis. The ROC curve analysis showed that the area under the curve (AUC) was 1 for both the NIR-SG1 and MRS ( p = 0.021 and p < 0.001, respectively), while the AUC = 0.910 for the Oil Red O stain ( p < 0.001) and the AUC = 0.865 for the CARS microscopy ( p < 0.001). The AUC for the 99m Tc MIBI SPECT was 0.640 ( p = 0.013), and this was a less discriminating technique for LS quantification.
Conclusions: The best-performing non-invasive methods for LS quantification are MRS, CARS microscopy, and the NIR-SG1. The NIR-SG1 is particularly appropriate for clinical practice and needs to be validated by clinical studies on liver grafts.
Databáze: MEDLINE
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