Floral Fusion: Unravelling the Potent Blend of Ixora coccinea and Rhododendron arboreum for Health and Safety Benefits.

Autor:	Bharathy P; Department of Pharmaceutics, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND., Thanikachalam PV; Department of Pharmaceutical Chemistry, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND., Shoban AN; Department of Pharmaceutics, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND., Himayavendhan HV; Department of Pharmaceutics, Saveetha College of Pharmacy, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Sep 23; Vol. 16 (9), pp. e70038. Date of Electronic Publication: 2024 Sep 23 (Print Publication: 2024).
DOI:	10.7759/cureus.70038
Abstrakt:	Objective: Ixora coccinea and Rhododendron arboreum are known for their traditional medicinal uses due to their diverse phytochemicals and pharmacological effects, which have attracted the interest of many researchers. This study aims to evaluate the antioxidant, anti-inflammatory, and cytotoxic effects of their combined extracts. Methods: In vitro antioxidant activity against reactive oxygen species (ROS) was measured using the ferric-reducing ability of plasma (FRAP), nitric oxide (NO), and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays, while anti-inflammatory effects were assessed via the membrane stabilization method. Docking studies were performed to evaluate the interaction of phytochemicals - anthocyanins, quercetin, and ursolic acid, which are present in these plants - with nuclear factor kappa B (NF-κB), cyclooxygenase-1 (COX-1), and cyclooxygenase-2 (COX-2). Standard protocols were used to evaluate embryotoxicity using the brine shrimp model and cytotoxicity using the zebrafish model, which is crucial for determining safe clinical dosages. Results: The analysis revealed diverse bioactive compounds, including anthocyanins, quercetin, and ursolic acid. The formulation effectively inhibited ROS production at lower concentrations (inhibitory concentration 50%, or IC50 value ~2.8 µg/mL), indicating their potential for managing oxidative stress. Quercetin demonstrated the strongest interaction with all tested proteins, particularly NF-kB. Cytotoxicity and embryotoxicity assays revealed a dose-dependent effect (lethal concentration 50%, or LC50 value 82.4 µg/mL), with no adverse effects on developing embryos at the tested doses (5-80 µg/mL), suggesting the extracts are safe for clinical use, even during pregnancy. Conclusion: The combined extracts of I. coccinea and R. arboreum exhibit potent antioxidant and anti-inflammatory effects without causing cytotoxic or embryotoxic effects, even at higher concentrations. This indicates their potential for safe clinical application in treating oxidative and inflammatory diseases. Competing Interests: Human subjects: All authors have confirmed that this study did not involve human participants or tissue. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Bharathy et al.)
Databáze:	MEDLINE
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