Evaluation of five different methods for diagnosis of Helicobacter pylori from fecal samples.

Autor: Horsma-Heikkinen J; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Pätäri-Sampo A; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Holma T; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Nevalainen A; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Friberg N; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Jarva H; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Loginov R; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland., Antikainen J; Department of Clinical Microbiology, Helsinki University Hospital, University of Helsinki, Helsinki, Finland.
Jazyk: angličtina
Zdroj: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2025 Jan; Vol. 133 (1), pp. e13483. Date of Electronic Publication: 2024 Oct 25.
DOI: 10.1111/apm.13483
Abstrakt: Accurate detection of Helicobacter pylori and its antimicrobial resistance is essential for eradication of the infections. The aim of this study was to compare five different CE-IVD marked assays in detection of H. pylori from 268 clinical stool samples. Samples were considered positive for H. pylori when at least three of the five tests were positive. Amplified IDEIA Hp StAR (Oxoid) and Premier Platinum HpSA PLUS (Meridian Bioscience Inc.) assays showed sensitivity of 100% [95% CI (confidence interval): 87-100] and LIAISON® Meridian H. pylori SA (DiaSorin) of 83.3% (95% CI: 66-93). Specificities of the assays were 94.5% (95% CI: 91-97), 95.4%; (95% CI: 92-97), and 97.1% (95% CI: 94-99) respectively. Amplidiag® H. pylori + ClariR (Mobidiag) assay showed 93.3% (95% CI: 78-99) and Allplex™ H. pylori & ClariR Assay (Seegene Inc.) 36.7% (95% CI: 22-55) sensitivity, while specificity of both was 97.9% (95% CI: 95-99). The Amplidiag® and Allplex™ assays concordantly detected clarithromycin resistance in positive for H. pylori samples. The Amplidiag® assay showed the highest accuracy, namely 97.4% (95% CI: 95-99). These data provide helpful information for planning laboratory diagnostics of H. pylori and detection of clarithromycin resistance from stool samples.
(© 2024 Scandinavian Societies for Pathology, Medical Microbiology and Immunology.)
Databáze: MEDLINE
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